Pharmacological analysis of 5-hydroxytryptamine actions on guinea-pig ileal mucosa.
Pharmacological analysis of 5-hydroxytryptamine actions on guinea-pig ileal mucosa.
复制标题
5-羟色胺对豚鼠回肠粘膜作用的药理学分析。
DOI：
10.1016/0014-2999(85)90639-9
复制
发表时间：
1985
影响因子：
5
通讯作者：
Carey,HV
中科院分区：
文献类型：
--
作者：
Cooke,HJ;Carey,HV
本实验在豚鼠回肠上研究了5-羟色胺（5-HT）参与电刺激粘膜下神经元引起的氯离子分泌反应的可能性。当电刺激脉冲的0.5 ms的持续时间，3.2 mA的振幅和频率为10 Hz的重复施加在平面的组织，在短路电流（Isc）的双相增加发生。快速耐受后，5-HT或5-HT拮抗剂西沙必利存在于洗澡溶液中时，肠神经的电刺激仍然引起了一个双相变化的Isc是相似的幅度前的反应，无论是治疗。西沙必利（5 μM）可防止粘膜对外源性5-HT的反应，而不改变组织的功能完整性。向组织的粘膜下侧添加100 μM 5-HT引起Isc的双相增加，这主要反映了氯化物分泌。河豚毒素和阿托品显着减少但不能消除Isc和氯化物分泌的变化。这些结果表明，由电场刺激引起的粘膜反应是不介导的释放5-HT在神经肠上皮细胞连接或从内分泌元件。5-羟色胺激活粘膜下神经丛内的肠胆碱能神经元，以刺激氯化物分泌。
The possibility that 5-hydroxytryptamine (5-HT) is involved in the chloride secretory response evoked by electrical stimulation of submucosal neurons was investigated in guinea-pig ileum set up in Ussing flux chambers. When electrical stimulus pulses of 0.5 ms duration, amplitude of 3.2 mA and frequency of 10 Hz were applied repetitively in the plane of the tissue, a biphasic increase in short-circuit current (Isc) occurred. After tachyphylaxis to 5-HT or when the 5-HT antagonist cisapride was present in the bathing solutions, electrical stimulation of enteric nerves still evoked a biphasic change in Isc that was similar in magnitude to the response before either treatment. Cisapride (5 μM) prevented the mucosal response to exogenous 5-HT without altering the functional integrity of the tissues. Addition of 100 μM 5-HT to the submucosal side of the tissue evoked a biphasic increase in Isc that reflected primarily chloride secretion. Tetrodotoxin and atropine significantly reduced but did not abolish the change in Isc and chloride secretion. These results suggest that the mucosal response evoked by electrical field stimulation is not mediated by release of 5-HT at neuro-enterocyte junctions or from endocrine elements. 5-Hydroxytryptamine activated enteric cholinergic neurons within the submucosal plexus to stimulate chloride secretion.