GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean.
GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean.
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GmWRKY31 和 GmHDL56 通过调节大豆防御相关基因表达增强对大豆疫霉的抗性
DOI：
10.3389/fpls.2017.00781
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发表时间：
2017
影响因子：
5.6
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Fan S;Dong L;Han D;Zhang F;Wu J;Jiang L;Cheng Q;Li R;Lu W;Meng F;Zhang S;Xu P
大豆疫霉根茎腐病[甘氨酸max (L.)]稳定。由大豆疫霉（Phytophthora sojae）引起，是一种世界性的破坏性疾病。大豆对黄豆病的分子反应机制在很大程度上尚不清楚。我们报道了一个新的WRKY转录因子（TF）在大豆中，GmWRKY31，在宿主对P. sojae的反应。过表达和RNA干扰分析表明，GmWRKY31增强了转基因大豆对大豆疫病的抗性。GmWRKY31靶向细胞核，与W-box结合，作为基因转录的激活因子。此外，我们确定GmWRKY31与GmHDL56物理互作，提高转基因大豆根系对大豆疫病的抗性。GmWRKY31和GmHDL56有一个共同的靶基因GmNPR1，是由大豆大豆蛋白酶诱导的。过表达和RNA干扰分析表明，GmNPR1增强了转基因大豆对大豆疫病的抗性。在过表达GmNPR1转录物的大豆植株中，几个致病相关基因（PR）被组成性激活，包括GmPR1a、GmPR2、GmPR3、GmPR4、GmPR5a和GmPR10。相比之下，在转基因GmNPR1-RNAi系中，PR基因的诱导受到损害。综上所述，GmWRKY31和GmHDL56的相互作用通过调节防御相关基因的表达来增强大豆对大豆卟卟菌的抗性。
Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by the oomycete Phytophthora sojae, is a destructive disease worldwide. The molecular mechanism of the soybean response to P. sojae is largely unclear. We report a novel WRKY transcription factor (TF) in soybean, GmWRKY31, in the host response to P. sojae. Overexpression and RNA interference analysis demonstrated that GmWRKY31 enhanced resistance to P. sojae in transgenic soybean plants. GmWRKY31 was targeted to the nucleus, where it bound to the W-box and acted as an activator of gene transcription. Moreover, we determined that GmWRKY31 physically interacted with GmHDL56, which improved resistance to P. sojae in transgenic soybean roots. GmWRKY31 and GmHDL56 shared a common target GmNPR1 which was induced by P. sojae. Overexpression and RNA interference analysis demonstrated that GmNPR1 enhanced resistance to P. sojae in transgenic soybean plants. Several pathogenesis-related (PR) genes were constitutively activated, including GmPR1a, GmPR2, GmPR3, GmPR4, GmPR5a, and GmPR10, in soybean plants overexpressing GmNPR1 transcripts. By contrast, the induction of PR genes was compromised in transgenic GmNPR1-RNAi lines. Taken together, these findings suggested that the interaction between GmWRKY31 and GmHDL56 enhances resistance to P. sojae by regulating defense-related gene expression in soybean.