Transcriptionally-active high-risk human papillomavirus is rare in oral cavity and laryngeal/hypopharyngeal squamous cell carcinomas - a tissue microarray study utilizing E6/E7 mRNA in situ hybridization
Transcriptionally-active high-risk human papillomavirus is rare in oral cavity and laryngeal/hypopharyngeal squamous cell carcinomas - a tissue microarray study utilizing E6/E7 mRNA in situ hybridization
复制标题
DOI：
10.1111/j.1365-2559.2011.04169.x
复制
发表时间：
2012-05-01
期刊：
影响因子：
6.4
通讯作者：
Chernock, Rebecca D.
中科院分区：
文献类型：
--
作者：
Lewis, James S., Jr.;Ukpo, Odey C.;Chernock, Rebecca D.
Lewis J S Jr, Ukpo O C, Ma X-J, Flanagan J J, Luo Y, Thorstad W L & Chernock R D (2012) 组织病理学 60, 982-991 转录活性高危人乳头瘤病毒在口腔和喉/下咽鳞状细胞癌中很少见 利用 E6/E7 mRNA 原位杂交的组织微阵列研究目的：人乳头瘤病毒在口咽鳞状细胞癌中已被确定为致病和预后因素，但其在非口咽肿瘤中的意义尚不清楚。特别是，寻找病毒 DNA 的重要性尚不清楚。我们试图评估非口咽鳞状细胞癌中具有转录活性的人乳头瘤病毒，并将其与病毒 DNA 的存在进行比较。方法：我们通过原位杂交评估了 87 例口腔和喉/下咽鳞状细胞癌患者的组织微阵列中的高危人乳头瘤病毒 DNA 和 E6 和 E7 mRNA 转录物，并通过免疫组织化学评估了 p16 表达。结果：我们发现 73 例可评估病例中只有 2 例 (2.7%) 携带具有转录活性的人乳头瘤病毒。这两个肿瘤均来自喉部，其中一个通过原位杂交显示人乳头瘤病毒 DNA 呈阳性，并且两者均呈 p16 广泛阳性。所有口腔和下咽肿瘤的人乳头瘤病毒呈阴性。结论：具有转录活性的人乳头瘤病毒在喉癌、下咽癌和口腔鳞状细胞癌中似乎很少见。因此，它似乎不太可能成为大多数已形成肿瘤的驱动因素或具有临床意义。
Lewis J S Jr, Ukpo O C, Ma X-J, Flanagan J J, Luo Y, Thorstad W L & Chernock R D (2012) Histopathology 60, 982-991 Transcriptionally-active high-risk human papillomavirus is rare in oral cavity and laryngeal/hypopharyngeal squamous cell carcinomas a tissue microarray study utilizing E6/E7 mRNA in situ hybridization Aims: Human papillomavirus is well established in oropharyngeal squamous cell carcinoma as both causative and prognostic, but its significance in non-oropharyngeal tumours is unclear. In particular, the significance of finding viral DNA is not known. We sought to evaluate nonoropharyngeal squamous cell carcinomas for transcriptionally-active human papillomavirus and to compare this with the presence of viral DNA. Methods: We evaluated an 87 patient tissue microarray cohort of oral cavity and laryngeal/hypopharyngeal squamous cell carcinomas for high risk human papillomavirus DNA and E6 and E7 mRNA transcripts by in situ hybridization, and for p16 expression by immunohistochemistry. Results: We found only two of the 73 (2.7%) evaluable cases to harbour transcriptionally-active human papillomavirus. Both of these tumours were from the larynx, one was positive for human papillomavirus DNA by in situ hybridization, and both were extensively positive for p16. All oral cavity and hypopharyngeal tumours were negative for human papillomavirus. Conclusions: Transcriptionally-active human papillomavirus appears to be rare in laryngeal, hypopharyngeal, and oral cavity squamous cell carcinomas. As such, it appears unlikely to be a driver or to be clinically significant in most established tumours.