Preservation of DNA Nanostructure Carriers: Effects of Freeze - Thawing and Ionic Strength during Lyophilization and Storage
Preservation of DNA Nanostructure Carriers: Effects of Freeze - Thawing and Ionic Strength during Lyophilization and Storage
复制标题
DOI：
10.1021/acsami.7b04784
复制
发表时间：
2017-06-07
影响因子：
9.5
通讯作者：
Wang, Lihua
中科院分区：
文献类型：
--
作者：
Zhu, Bing;Zhao, Yan;Wang, Lihua
DNA结构在生物医学应用中引起了广泛的兴趣，特别是作为药物输送的纳米载体。因此，它是。重要的是要确保：DNA纳米结构在室温储存下的结构完整性。在这项研究中，我们通过研究冻干后重建的不同DNA纳米结构的结构完整性来研究基于冻干的DNA纳米结构保存。我们证明了在适当的离子强度下冻干有利于DNA的储存。经过相当于100天室温贮藏的加速老化试验，冻干重组的DNA纳米结构载体的结构完整性明显优于液体溶液。
DNA rianostiuctures have: attracted wide interest-in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is.important to ensure:the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization Under appropriate ionic strength is amenable to the storage of DNA. nanostructures Compared with that stored in liquid solutiOn, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.