Expression of the S-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector.
Expression of the S-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector.
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使用杆状病毒载体表达淋巴细胞性脉络丛脑膜炎沙粒病毒的 S 编码基因。
DOI：
10.1099/0022-1317-67-8-1515
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
D. Bishop
中科院分区：
文献类型：
--
作者：
Y. Matsuura;R. Possee;D. Bishop
淋巴细胞性脉络丛脑膜炎病毒（LCMV，WE 株）S RNA 种类的 DNA 拷贝已以两个方向插入到含有杆状病毒苜蓿银纹夜蛾核型多角体病毒 (AcNPV) 的 7.1 kb DNA 序列的质粒中。插入片段被放置在AcNPV的多角体蛋白基因启动子后面，并且位于包括多角体蛋白基因翻译起始密码子及其侧翼的核苷酸序列的位置。在感染性 AcNPV DNA 存在下转染草地贪夜蛾细胞并选择多角体蛋白阴性病毒后，使用衍生的质粒获得重组 AcNPV 病毒。描述了重组杆状病毒在草地贪夜蛾细胞中表达两种 LCMV S 编码基因（核蛋白和糖蛋白前体）。基于用三种不同表达载体获得的结果，外源基因在多角体蛋白基因的5'非编码区中的插入位点似乎是获得的表达水平的重要决定因素。
A DNA copy of the lymphocytic choriomeningitis virus (LCMV, WE strain)S RNA species has been inserted in both orientations into plasmids containing a 7.1 kb DNA sequence of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The inserts were placed behind the polyhedrin gene promoter of AcNPV and in the place of the nucleotide sequences including and flanking the polyhedrin gene translation initiation codon. The derived plasmids were used to obtain recombinant AcNPV viruses after transfection of Spodoptera frugiperda cells in the presence of infectious AcNPV DNA and the selection of polyhedrin-negative viruses. The expression of the two LCMV S-coded genes, the nucleoprotein and glycoprotein precursor, in S. frugiperda cells by the recombinant baculoviruses is described. Based on the results obtained with three different expression vectors, the site of insertion of the foreign genes in the 5' non-coding region of the polyhedrin gene appears to be an important determinant of the level of expression obtained.