VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro.
VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro.
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VCAM-1靶向核/壳纳米颗粒在体外剪切流和细胞磁共振成像下选择性粘附并递送至脂多糖诱导炎症的内皮细胞
DOI：
10.2147/ijn.s44997
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发表时间：
2013
影响因子：
8
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Yang H;Zhao F;Li Y;Xu M;Li L;Wu C;Miyoshi H;Liu Y
多功能纳米材料具有独特的磁性和发光性能，在生物学领域具有广阔的应用前景。由于与正常内皮细胞相比，炎性内皮细胞中血管细胞粘附分子-1（VCAM-1）受体过表达，因此抗VCAM-1单克隆抗体可用作靶向配体。在本文中，我们描述了多功能核-壳Fe3O4@SiO2纳米颗粒的开发，其具有通过VCAM-1、磁性和荧光成像靶向炎症内皮细胞的能力，具有有效的磁共振成像对比度特性。将超顺磁性氧化铁和异硫氰酸荧光素（FITC）分别成功地装载在纳米颗粒核和二氧化硅壳内，产生VCAM-1靶向的Fe3O4@SiO2（FITC）纳米颗粒，其通过扫描电子显微镜、透射电子显微镜、荧光光谱法、zeta电位测定和荧光显微镜进行表征。VCAM-1靶向的Fe 3 O4@SiO2（FITC）纳米颗粒的直径通常为355 ± 37 nm，在室温下表现出超顺磁性行为，并与脂多糖激活的人脐静脉内皮细胞（HUVEC-CS）的炎症亚系累积和靶向粘附。此外，我们的数据显示，VCAM-1靶向Fe3O4@SiO2（FITC）纳米颗粒与炎症HUVEC-CS的粘附取决于剪切应力和暴露于应力的持续时间。对HUVEC-CS内化的分析表明，VCAM-1靶向Fe3O4@SiO2（FITC）纳米颗粒的递送效率也显著高于非靶向Fe3O4@SiO2（FITC）-NH 2纳米颗粒。磁共振图像显示，VCAM-1靶向Fe3O4@SiO2（FITC）纳米颗粒的超顺磁性氧化铁核心也可以作为磁共振成像的造影剂。综上所述，这些VCAM-1靶向Fe3O4@SiO2（FITC）纳米颗粒靶向炎症内皮细胞的累积粘附和摄取潜力可用于将治疗药物/基因转移到这些细胞中，或用于未来在分子和细胞水平上诊断血管疾病。
Multifunctional nanomaterials with unique magnetic and luminescent properties have broad potential in biological applications. Because of the overexpression of vascular cell adhesion molecule-1 (VCAM-1) receptors in inflammatory endothelial cells as compared with normal endothelial cells, an anti-VCAM-1 monoclonal antibody can be used as a targeting ligand. Herein we describe the development of multifunctional core-shell Fe3O4@SiO2 nanoparticles with the ability to target inflammatory endothelial cells via VCAM-1, magnetism, and fluorescence imaging, with efficient magnetic resonance imaging contrast characteristics. Superparamagnetic iron oxide and fluorescein isothiocyanate (FITC) were loaded successfully inside the nanoparticle core and the silica shell, respectively, creating VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles that were characterized by scanning electron microscopy, transmission electron microscopy, fluorescence spectrometry, zeta potential assay, and fluorescence microscopy. The VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles typically had a diameter of 355 ± 37 nm, showed superparamagnetic behavior at room temperature, and cumulative and targeted adhesion to an inflammatory subline of human umbilical vein endothelial cells (HUVEC-CS) activated by lipopolysaccharide. Further, our data show that adhesion of VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles to inflammatory HUVEC-CS depended on both shear stress and duration of exposure to stress. Analysis of internalization into HUVEC-CS showed that the efficiency of delivery of VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles was also significantly greater than that of nontargeted Fe3O4@SiO2(FITC)-NH2 nanoparticles. Magnetic resonance images showed that the superparamagnetic iron oxide cores of the VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles could also act as a contrast agent for magnetic resonance imaging. Taken together, the cumulative adhesion and uptake potential of these VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles targeted to inflammatory endothelial cells could be used in the transfer of therapeutic drugs/genes into these cells or for diagnosis of vascular disease at the molecular and cellular levels in the future.