Rapid diagnosis of varicella-zoster virus infections by countercurrent immunoelectrophoresis.
Rapid diagnosis of varicella-zoster virus infections by countercurrent immunoelectrophoresis.
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通过逆流免疫电泳快速诊断水痘带状疱疹病毒感染。
DOI：
10.1093/infdis/143.2.274
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Gershon,AA
中科院分区：
文献类型：
--
作者：
Frey,HM;Steinberg,SP;Gershon,AA
描述了一种使用逆流免疫电泳 (CIE) 进行水痘带状疱疹病毒 (VZV) 感染实验室诊断的简单、准确和快速的方法。 CIE 使用带状疱疹恢复期血清检测水疱液中的 VZV 抗原。研究对象为 86 名患者，其中 58 名患有水痘带状疱疹病毒感染，28 名患者因水痘带状疱疹病毒感染以外的原因出现水疱或大疱性皮疹。所有记录有水痘带状疱疹病毒感染的患者的水痘带状疱疹病毒 CIE 检测均呈阳性，而对照组均呈阴性。皮疹出现后 15 天（水痘）或 16 天（带状疱疹）可检测到 VZV 抗原。将 CIE 检测 VZV 抗原的灵敏度与病毒分离、免疫粘附血凝和间接酶联免疫吸附测定的结果进行比较。
A simple, accurate, and rapid method for laboratory diagnosis of varicella-zoster virus (VZV) infections using countercurrent immunoelectrophoresis (CIE) is described. CIE uses zoster convalescent-phase serum to detect VZV antigen in vesicular fluid. Eighty-six patients were studied, 58 with VZV infections and 28 with vesicular or bullous rashes due to causes other than VZV infection. All patients with documented VZV infection had positive CIE tests for VZV, and the controls were uniformly negative. VZV antigen could be detected up to 15 (varicella) or 16 (zoster) days after the onset of rash. The sensitivity of CIE for detection of VZV antigen was compared with results of viral isolation, immune adherence hemagglutination, and an indirect enzyme-linked immunosorbent assay.