Skeletal muscle sarcoplasmic reticulum in porcine malignant hyperthermia.
Skeletal muscle sarcoplasmic reticulum in porcine malignant hyperthermia.
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猪恶性高热中的骨骼肌肌浆网。
DOI：
10.1016/0014-2999(79)90010-4
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发表时间：
1979
影响因子：
5
通讯作者：
S. R. Taylor
中科院分区：
文献类型：
--
作者：
G. Gronert;J. Heffron;S. R. Taylor
为探讨肌浆网(SR)在恶性高热中的作用，从正常和遗传易感的波兰中国猪的半腱肌肌中分离到肌浆网。测定包括钙结合率(无草酸盐)、钙摄取率和钙摄取量(草酸盐存在)，以及使用和不使用氟烷时的自发钙释放(在没有离子去极化或钙的情况下)，使用微孔过滤技术。与正常猪相比，易感猪SR片段的钙结合率、钙摄取率和钙摄取量降低，自发钙释放增加。0.5%氟烷使易感SR和正常SR的钙结合率略有增加。在1%以上，氟烷降低敏感SR和正常SR的钙结合率、摄取率和摄取量，增加钙释放。这些SR功能的差异不足以解释恶性高热的病因，氟烷的作用也不能解释其触发作用。
To examine the function of sarcoplasmic reticulum (SR) in malignant hyperthermia, SR was isolated from semitendinosus muscle of normal and genetically susceptible Poland China swine. Determinations included rate of calcium binding (oxalate absent), rate and capacity of calcium uptake (oxalate present), and spontaneous calcium release (in the absence of ionic depolarization or calcium) with and without halothane, using the millipore filtration technique. Rate of calcium binding, and rate and capacity of calcium uptake were decreased, and spontaneous calcium release was greater in SR fragments from susceptible swine as compared to those from normal swine. Halothane 0.5% slightly increased the rate of calcium binding in susceptible and normal SR. Above 1%, halothane decreased calcium binding rate, and uptake rate and capacity, and increased calcium release similarly in susceptible and normal SR. These differences in SR function were insufficient to explain the etiology of malignant hyperthermia, nor did the effect of halothane account for its triggering action.