ATP-dependent and cyclic AMP-dependent activation of rat adipose tissue lipase by protein kinase from rabbit skeletal muscle.
ATP-dependent and cyclic AMP-dependent activation of rat adipose tissue lipase by protein kinase from rabbit skeletal muscle.
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兔骨骼肌蛋白激酶对大鼠脂肪组织脂肪酶的 ATP 依赖性和环 AMP 依赖性激活。
DOI：
10.1073/pnas.67.1.290
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发表时间：
1970
影响因子：
11.1
通讯作者：
S. Mayer
中科院分区：
文献类型：
--
作者：
J. Huttunen;D. Steinberg;S. Mayer
从大鼠附睾脂肪垫中提取的部分纯化的激素敏感脂肪酶制剂与ATP、Mg（++）、环腺苷3′：5′-单磷酸和兔肌蛋白激酶（磷酸化酶b激酶）短暂孵育后，溶脂活性增强（44-93%）。当忽略辅因子混合物或蛋白激酶时，观察到很少或没有激活。当脂肪垫在匀浆前与肾上腺素孵育时，向可溶性上清部分添加激酶和辅助因子不会引起活化，而在未暴露于肾上腺素的成对脂肪垫制备的制剂中获得了良好的活化。结果表明，环amp介导的脂肪组织中激素敏感脂肪酶的激活涉及蛋白质磷酸化步骤。脂肪酶本身是否被磷酸化从而被激活，或者蛋白激酶是否像其在糖原磷酸化酶系统中的作用一样激活了一种中介酶，仍有待确定。
Brief incubation of partially purified preparations of hormone-sensitive lipase from rat epididymal fat pads with ATP, Mg(++), cyclic adenosine 3':5'-monophosphate and rabbit muscle protein kinase (phosphorylase b kinase kinase) resulted in enhancement of lipolytic activity (44-93%). Little or no activation was observed when either the cofactor mixture or the protein kinase was omitted. When the fat pads were incubated with epinephrine prior to homogenization, addition of kinase and cofactors to the soluble supernatant fraction caused no activation whereas good activation was obtained in preparations from paired fat pads not exposed to epinephrine. The results indicate that the cyclic AMP-mediated activation of hormone-sensitive lipase in adipose tissue involves a protein phosphorylation step. Whether the lipase itself is phosphorylated and thus activated or whether the protein kinase is activating a mediating enzyme, in analogy with its action in the glycogen phosphorylase system, remains to be determined.