Polarization-based sensing with a self-referenced sample
Polarization-based sensing with a self-referenced sample
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DOI：
10.1366/0003702991947964
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发表时间：
1999-09-01
影响因子：
3.5
通讯作者：
Rao, G
中科院分区：
文献类型：
--
作者：
Lakowicz, JR;Gryczynski, I;Rao, G
介绍了一种新的基于荧光偏振的荧光传感方法，该传感器由TNO隔室组成，TNO隔室和TNO隔室均含有传感荧光团。传感器的一侧包含恒定浓度的分析物，另一侧包含未知浓度。两边的发射通过偏振器观察，样品的偏振比参照物的偏振旋转90度。样品荧光强度的变化会导致组合发射的测量偏振的变化。我们表明，这种方法可以用来测量葡萄糖和钙使用荧光团，这显示了分析物依赖的强度变化，而不是光谱形状的变化。只需要一个荧光团，这就是传感器两侧的感测荧光团。我们还表明，葡萄糖和钙的偏振传感可以通过对偏振的目测来执行。在这种情况下，唯一的电子元件是光源。这些简单的方案可以与各种分析物一起使用，唯一的要求是改变荧光强度以响应分析物。
We describe a new method of fluorescence sensing based on fluorescence polarization, The sensor consists of tno compartments, both of which contain the sensing fluorophore. One side of the sensor contains a constant concentration of analyte, and the other contains the unknown concentration. Emission from both sides is observed through polarizers, with the polarization from the sample being rotated 90 degrees from that of the reference. Changes in the fluorescence intensity of the sample result in changes in the measured polarization for the combined emission. We show that this approach can be used to measure glucose and calcium using fluorophores which show analyte-dependent intensity changes, and no change in the spectral shape. Only a single fluorophore is required, this being the sensing fluorophore in both sides of the sensor. We also show that polarization sensing of glucose and calcium can be performed with visual detection of the polarization. In this case the only electronic component is the light source. These simple schemes can be used with a variety of analytes, The only requirement is a change in Fluorescence intensity in response to the analyte.