Self-Regulating Enzyme-Nanotube Ensemble Films and Their Application as Flexible Electrodes for Biofuel Cells
Self-Regulating Enzyme-Nanotube Ensemble Films and Their Application as Flexible Electrodes for Biofuel Cells
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DOI：
10.1021/ja111517e
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发表时间：
2011-04-06
影响因子：
15
通讯作者：
Nishizawa, Matsuhiko
中科院分区：
文献类型：
--
作者：
Miyake, Takeo;Yoshino, Syuhei;Nishizawa, Matsuhiko
纳米结构碳由于其大的比表面积而被广泛用于制备酶修饰电极。然而，由于它们是特定或管状纳米碳的随机聚集体，因此酶对其intranospace的后修饰通常难以控制。在这里，我们描述了一个独立的碳纳米管森林（CNTF），可以通过液体诱导收缩与酶形成一个混合系综膜。这提供了原位调节其纳米内空间（碳纳米管间距）的酶的大小，并最终作为一个高活性的电极。CNTF与果糖脱氢酶（FDH）的系综在搅拌的200 mM果糖溶液中显示16 mA cm（-2）的氧化电流密度。以FDH-CNTF为阳极，漆酶CNTF为阴极的生物燃料电池在搅拌的含氧果糖溶液中的功率密度达到1.8mW cm（-2）（0.45 V），连续运行24 h后，功率密度仍保持在80%以上。应用程序的独立，灵活的字符的酶CNTF合奏电极证明通过其使用的补丁或伤口的形式。
Nanostructured carbons have been widely used for fabricating enzyme-modified electrodes due to their large specific surface area. However, because they are random aggregates of particular or tubular nanocarbons, the postmodification of enzymes to their intrananospace is generally hard to control. Here, we describe a free-standing film of carbon nanotube forest (CNTF) that can form a hybrid ensemble with enzymes through liquid-induced shrinkage. This provides in situ regulation of its intrananospace (inter-CNT pitch) to the size of enzymes and eventually serves as a highly active electrode. The CNTF ensemble with fructose dehydrogenase (FDH) showed the oxidation current density of 16 mA cm(-2) in stirred 200 mM fructose solution. The power density of a biofuel cell using the FDH-CNTF anode and the Laccase CNTF cathode reached 1.8 mW cm(-2) (at 0.45 V) in the stirred oxygenic fructose solution, more than 80% of which could be maintained after continuous operation for 24 h. Application of the free-standing, flexible character of the enzyme CNTF ensemble electrodes is demonstrated via their use in the patch or wound form.