Parkinson's disease involves autophagy and abnormal distribution of cathepsin L
Parkinson's disease involves autophagy and abnormal distribution of cathepsin L
复制标题
DOI：
10.1016/j.neulet.2010.11.068
复制
发表时间：
2011-02-01
影响因子：
2.5
通讯作者：
Liang, Zhongqin
中科院分区：
文献类型：
--
作者：
Li, Lingyun;Wang, Xiaoxia;Liang, Zhongqin
Accumulating evidences suggest that the related autophagy-lysosomal mechanism plays a critical role in many neurodegenerative disorders. In this study, we examined postmortem Parkinson's disease (PD) substantia nigra for evidence of cathepsin L by immunofluorescent staining, and found increased expression of cathepsin L in dopamine neurons of PD patients. We confirmed 6-OHDA induced nuclear translocation of cathepsin L in rat substantia nigral neurons as well. Furthermore, we observed autophagic vacuoles and lysosomes were accumulated in the 6-hydroxydopamine (6-OHDA) injured rat substantia nigra neurons with electron microscopy. Immunofluorescent staining showed that LC3 was enriched in dopamine neurons after 6-OHDA treatment. When pretreated with 3-methyladenine (3-MA), dopaminergic neurons were protected from cell death induced by 6-OHDA, associated with the suppression of LC3 and cathepsin L Our results demonstrate that activation of autophagy and abnormal distribution of cathepsin L may be responsible for dopamine neuron death, involved in the pathogenic cascade event for the development of Parkinson's disease. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
