Function of the Carbohydrate Moieties of Glycoproteins
Function of the Carbohydrate Moieties of Glycoproteins
复制标题
糖蛋白碳水化合物部分的功能
DOI：
10.1002/jcb.1982.240180306
复制
发表时间：
1982
影响因子：
4
通讯作者：
J. Parent
中科院分区：
文献类型：
--
作者：
K. Olden;B. Bernard;S. White;J. Parent
为了确定糖蛋白的碳水化合物部分的功能，我们使用衣霉素（N-乙酰葡糖胺的类似物）来抑制N-糖苷连接的糖蛋白的糖基化。首先，我们研究了这种药物对鸡胚成纤维细胞的主要细胞表面糖蛋白纤维连接蛋白的细胞内加工、输出和生物学活性的影响。用衣霉素处理的鸡成纤维细胞仅产生非糖基化纤连蛋白，并且无碳水化合物蛋白种类的输出或分泌未完全受损。我们确实观察到细胞表面和培养基中非糖基化纤连蛋白的绝对量显著减少。这种降低是由于非糖基化蛋白质种类的蛋白水解降解增加所致。
To determine the function of the carbohydrate moiety of glycoproteins, we have used tunicamycin, an analog of N‐acetylglucosamine, to inhibit the glycosylation of N‐glycosidically linked glycoproteins. First, we examined the effect of this drug on the intracellular processing, export and biological activity of fibronectin–the major cell surface glycoprotein of chick embryo fibroblasts. Chick fibroblasts treated with tunicamycin produced only nonglycosylated fibronectin and the export or secretion of the carbohydrate‐free protein species was not totally impaired. We did observe that there was a substantial decrease in the absolute amount of nonglycosylated fibronectin on the cell surface and in the culture medium. This decrease was shown to be due to increased proteolytic degradation of the nonglycosylated protein species.
大鼠肝脏中的磷酸甘露糖基酶受体。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fischer,HD;Gonzalez-Noriega,A;Sly,WS;Morré,DJ
通讯作者：
Morré,DJ