Viral metagenomic analysis of feces of wild small carnivores.
Viral metagenomic analysis of feces of wild small carnivores.
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DOI：
10.1186/1743-422x-11-89
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发表时间：
2014-05-15
期刊：
影响因子：
4.8
通讯作者：
Smits SL
中科院分区：
文献类型：
--
作者：
Bodewes R;Ruiz-Gonzalez A;Schapendonk CM;van den Brand JM;Osterhaus AD;Smits SL
最近的研究清楚地表明，野生动物中存在着巨大的病毒多样性。这表明，我们需要进一步了解野生动物中存在的病毒多样性，以及这些病毒可能向家畜或人类传播的情况。在本研究中，我们利用随机PCR结合新一代测序技术，对西班牙北部10种不同野生小型食肉动物粪便样本（n = 42）的病毒多样性进行了评估。采集鼬科美洲貂（Neovison vison）、欧洲貂（Mustela lutreola）、欧洲鼬（Mustela putorius）、欧洲松貂（Martes Martes）、石貂（Martes foina）、欧亚水獭（Lutra Lutra）和欧亚獾（Meles Meles）的标本；蚤科的普通蚤（蚤属）；狐科的赤狐（Vulpes Vulpes）和狐科的欧洲野猫（Felis silvestris）。检测到许多可能的新型病毒或病毒变体序列，包括他们的病毒、静脉病毒、耳病毒、科布病毒和小核糖核酸病毒。采用随机PCR与下一代测序相结合的方法，在西班牙食肉动物粪便样本中检测到各种新型病毒或病毒变体的序列。检测到的新型病毒突出了野生食肉动物粪便中存在的病毒多样性。
Recent studies have clearly demonstrated the enormous virus diversity that exists among wild animals. This exemplifies the required expansion of our knowledge of the virus diversity present in wildlife, as well as the potential transmission of these viruses to domestic animals or humans. In the present study we evaluated the viral diversity of fecal samples (n = 42) collected from 10 different species of wild small carnivores inhabiting the northern part of Spain using random PCR in combination with next-generation sequencing. Samples were collected from American mink (Neovison vison), European mink (Mustela lutreola), European polecat (Mustela putorius), European pine marten (Martes martes), stone marten (Martes foina), Eurasian otter (Lutra lutra) and Eurasian badger (Meles meles) of the family of Mustelidae; common genet (Genetta genetta) of the family of Viverridae; red fox (Vulpes vulpes) of the family of Canidae and European wild cat (Felis silvestris) of the family of Felidae. A number of sequences of possible novel viruses or virus variants were detected, including a theilovirus, phleboviruses, an amdovirus, a kobuvirus and picobirnaviruses. Using random PCR in combination with next generation sequencing, sequences of various novel viruses or virus variants were detected in fecal samples collected from Spanish carnivores. Detected novel viruses highlight the viral diversity that is present in fecal material of wild carnivores.