INTERACTION OF DURAMYCIN WITH ARTIFICIAL AND NATURAL MEMBRANES
INTERACTION OF DURAMYCIN WITH ARTIFICIAL AND NATURAL MEMBRANES
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DOI：
10.1021/bi00338a025
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
RACKER, E
中科院分区：
文献类型：
--
作者：
NAVARRO, J;CHABOT, J;RACKER, E
耐久霉素是一种多肽抗生素（分子量 2012），从 Streptomyces cinnamomeus forma azacoluta 的培养滤液中获得。在这项工作中，我们证明低浓度的耐久霉素诱导含有不饱和磷脂酰乙醇胺和不饱和单半乳糖基甘油二酯的脂质囊泡以及来自兔骨骼肌的肌浆网囊泡的聚集。此外，耐久霉素抑制肌浆网囊泡中 ATP 依赖性 Ca2+ 摄取，而不影响 ATP 水解或 Ca2+ 通透性。此外，耐久霉素仅抑制重组为含有磷脂酰乙醇胺的磷脂囊泡的细菌视紫红质质子泵。我们分离出了一株耐久霉素耐药的枯草芽孢杆菌菌株，并绘制了耐久霉素耐药的位置图。在该菌株中，质子的分泌和钙的流入对耐久霉素具有抗性，并且其脂质组成与亲本菌株有很大不同。耐药菌株中未检测到磷脂酰乙醇胺。我们的研究结果与耐久霉素识别由磷脂酰乙醇胺或单半乳糖甘油二酯的存在决定的特定膜构象的观点是一致的。
Duramycin is a polypeptide antibiotic (molecular weight 2012) obtained from culture filtrates of Streptomyces cinnamomeus forma azacoluta. In this work, we show that low concentrations of duramycin induced aggregation of lipid vesicles containing unsaturated phosphatidylethanolamine and unsaturated monogalactosyl diglyceride, and of sarcoplasmic reticulum vesicles from rabbit skeletal muscle. Furthermore, duramycin inhibited the ATP-dependent Ca2+ uptake in sarcoplasmic reticulum vesicles without affecting the hydrolysis of ATP or the permeability of Ca2+. Also, duramycin only inhibited the bacteriorhodopsin proton pump reconstituted into phospholipid vesicles containing phosphatidylethanolamine. We have isolated a duramycin-resistant strain of Bacillus subtilis and have mapped the location of duramycin resistance. In this strain, the secretion of protons and influx of calcium were resistant to duramycin, and its lipid composition was profoundly different from that of the parent strain. No phosphatidylethanolamine was detected in the resistant strain. Our findings are consistent with the idea that duramycin recognizes a particular membrane conformation determined by the presence of phosphatidylethanolamine or monogalactosyl diglyceride.