IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MAN
IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MAN
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DOI：
10.1172/jci104130
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发表时间：
1960-01-01
影响因子：
15.9
通讯作者：
BERSON, SA
中科院分区：
文献类型：
--
作者：
YALOW, RS;BERSON, SA
本文报道了人胰岛素与牛胰岛素-IL-31竞争免疫豚鼠血清中胰岛素结合抗体的反应，建立了测定人血浆胰岛素的免疫分析法。该方法对小于1 [mu]V的胰岛素敏感，允许测量10至20 [mu]l血浆中的胰岛素浓度。体外加入血浆中的人胰岛素可定量回收，在1：2至1：100范围内，测定的内源性胰岛素浓度随血浆稀释度成比例降低。内源性血浆胰岛素通过与半胱氨酸孵育而被破坏，并且纤维素柱对内源性胰岛素的吸附在数量上类似于添加的牛肉胰岛素-I131的吸附。使用不同批次的胰岛素-1131，间隔一个月或多个月重复测定相同血浆样本（中间冷冻储存）的胰岛素浓度，结果通常一致。列出了非糖尿病和糖尿病人类受试者的胰岛素浓度数据。根据所提供的数据，计算平均正常每日胰岛素分泌率，”估计每天约有55 U胰岛素。
An immunoassay for plasma insulin in man is presented, based on the reaction of human insulin, competing with beef insulin-Il31, with insulin-binding antibodies in the sera of guinea pigs immunized with beef insulin. The method is sensitive to less than 1 [mu]V of insulin, permitting measurement of insulin concentrations in 10 to 20 [mu]l of plasma. Human insulin added in vitro to plasma is recovered quantitatively, and measured endogenous insulin concentrations decrease proportionately on dilution of plasma over the range 1:2 to 1:100. Endogenous plasma insulin is destroyed by incubation with cysteine and endogenous insulin adsorption by cellulose columns is quantitatively similar to the adsorption of added beef insulin-I131. Repeat determinations of insulin concentrations on the same plasma samples (stored frozen in the interim) one or more months apart, with different lots of insulin-1131, were generally in good agreement. Data are presented for insulin concentrations in nondiabetic and diabetic human subjects. Calculation of the average normal daily insulin secretion rate, on the basis of the data presented," yields an estimate of about 55 U of insulin per day.