Synergetic Effects of K, Ca, Cu and Zn in Human Semen in Relation to Parameters Indicative of Spontaneous Hyperactivation of Spermatozoa
Synergetic Effects of K, Ca, Cu and Zn in Human Semen in Relation to Parameters Indicative of Spontaneous Hyperactivation of Spermatozoa
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DOI：
10.1371/journal.pone.0152445
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发表时间：
2016-03-31
期刊：
影响因子：
3.7
通讯作者：
Kuna, Krunoslav
中科院分区：
文献类型：
--
作者：
Bolanca, Ivan;Obhodas, Jasmina;Kuna, Krunoslav
我们已经观察到，与接受常规体外受精的精子活力降低的男性组相比，精子质量参数指示精子超活化，例如较低的“线性”和“直线度”，以及如本研究所示的“伸长”，在精子图正常的患者中更明显。该研究包括97名被诊断为正常精子症（n = 20）、弱精子症（n = 54）和少弱精子症（n = 23）的男性。研究结果表明，根据WHO标准诊断的正常精子图患者的精子质量可能会受到影响，表现出过早的自发性超激活，这可能会降低自然受孕的机会。我们评估了射精精液中多种化学元素的协同作用，以发现精子的过早自发超活化是否可能是精液成分不平衡的标志，尤其是元素K，Ca，Cu和Zn。在所有三个诊断组的样本中发现了显示低或高基线状态的化学元素浓度的人类精液样本。然而，考虑到所有三组男性的样本，K/Ca和Cu/Zn比值的相关性在统计学显著性水平p = 0.01时为负相关。我们测试了K/Ca和Cu/Zn比值之间的负相关性是否适用于更大数量的精液样本。我们发现，负相关是有效的175精液样本的统计学意义的p = 0.00002。K/Ca和Cu/Zn的比值，即K和Zn的浓度相对于Ca和Cu的浓度增加，与具有正常精子图和明显的精子自发性过度活化的男性组中的“直性”降低相关，这意味着这些元素协同作用，并且元素的平衡而不是它们的绝对浓度在过早精子超活化中起主要作用在射出的精液中
We have observed that sperm quality parameters indicative of spermatozoa hyperactivation such are lower "linearity" and "straightness", and as showed by this research "elongation", were more pronounced in patients with normal spermiogram compared to the group of men with reduced sperm motility who were undergoing routine in vitro fertilisation. The research encompassed 97 men diagnosed with normozoospermia (n = 20), asthenozoospermia (n = 54) and oligoasthenozoospermia (n = 23). The findings indicate that sperm quality of patients with normal spermiogram diagnosed according to WHO criteria, may be compromised by showing premature spontaneous hyperactivation which can decrease the chances of natural conception. We assessed synergistic effects of multiple chemical elements in ejaculated semen to find if premature spontaneous hyperactivation of spermatozoa can be a sign of imbalanced semen composition especially of elements K, Ca, Cu and Zn. Human semen samples showing low or high baseline status of chemical elements concentrations were found in samples from all three diagnostic groups. However, correlation of K/Ca and Cu/Zn ratios, taking into account samples from all three groups of men, were negative at statistical significance level p = 0.01. We tested if the negative correlation between K/Ca and Cu/Zn ratio works for greater number of semen samples. We found the negative correlation to be valid for 175 semen samples at statistical significance of p = 0.00002. The ratio of K/Ca and Cu/Zn, i.e. increased concentrations of K and Zn in comparison to concentrations of Ca and Cu, were associated with a decrease of "straightness" in the group of men with normal spermiogram and pronounced spontaneous hyperactivation of spermatozoa, implying that these elements act in synergy and that the balance of elements and not their absolute concentrations plays the major role in premature spermatozoa hyperactivation in ejaculated semen.