Hippocampal hub neurons maintain distinct connectivity throughout their lifetime
Hippocampal hub neurons maintain distinct connectivity throughout their lifetime
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DOI：
10.1038/s41467-020-18432-6
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发表时间：
2020-09-11
影响因子：
16.6
通讯作者：
Cossart, Rosa
中科院分区：
文献类型：
--
作者：
Bocchio, Marco;Gouny, Claire;Cossart, Rosa
皮层GABA神经元的颞叶胚胎起源对其特化至关重要。在新生儿海马体中，最早出生的GABA细胞（ebGABAs）通过协调群体同步而作为“枢纽”运作。然而，他们成年后的命运在很大程度上仍然未知。为了填补这一空白，我们已经检查了CA1 ebGABAs使用电生理学，神经化学分析，光遗传学连接映射以及离体和体内钙成像的组合。我们发现，CA1 ebGABAs不仅在发展过程中作为枢纽，但也保持不同的形态生理和连接配置文件，包括远程目标和本地兴奋性输入的偏见。在体内，ebGABA在运动过程中被激活，与CA1细胞组装相关，并显示出高功能连接。因此，ebGABA从出生起就被指定，以确保其一生中的独特功能。在成年人的大脑中，这可能通过协调遥远区域的细胞组装来发挥远程枢纽作用。
The temporal embryonic origins of cortical GABA neurons are critical for their specialization. In the neonatal hippocampus, GABA cells born the earliest (ebGABAs) operate as `hubs' by orchestrating population synchrony. However, their adult fate remains largely unknown. To fill this gap, we have examined CA1 ebGABAs using a combination of electrophysiology, neurochemical analysis, optogenetic connectivity mapping as well as ex vivo and in vivo calcium imaging. We show that CA1 ebGABAs not only operate as hubs during development, but also maintain distinct morpho-physiological and connectivity profiles, including a bias for long-range targets and local excitatory inputs. In vivo, ebGABAs are activated during locomotion, correlate with CA1 cell assemblies and display high functional connectivity. Hence, ebGABAs are specified from birth to ensure unique functions throughout their lifetime. In the adult brain, this may take the form of a long-range hub role through the coordination of cell assemblies across distant regions.