RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast
RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast
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DOI：
10.1016/j.molcel.2018.10.046
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发表时间：
2019-01-17
期刊：
影响因子：
16
通讯作者：
Henikoff, Steven
中科院分区：
文献类型：
--
作者：
Brahma, Sandipan;Henikoff, Steven
The classic view of nucleosome organization at active promoters is that two well-positioned nucleo-somes flank a nucleosome-depleted region (NDR). However, this view has been recently disputed by contradictory reports as to whether wider (greater than or similar to 150 bp) NDRs instead contain unstable, micrococcal nuclease-sensitive ("fragile'') nucleosomal particles. To determine the composition of fragile particles, we introduce CUT&RUN. ChIP, in which targeted nuclease cleavage and release is followed by chromatin immunoprecipitation. We find that fragile particles represent the occupancy of the RSC (remodeling the structure of chromatin) nucleosome remodeling complex and RSC-bound, partially unwrapped nucleosomal intermediates. We also find that general regulatory factors (GRFs) bind to partially unwrapped nucleosomes at these promoters. We propose that RSC binding and its action cause nucleosomes to unravel, facilitate subsequent binding of GRFs, and constitute a dynamic cycle of nucleosome deposition and clearance at the subset of wide Pol II启动子 NDR。
The classic view of nucleosome organization at active promoters is that two well-positioned nucleo-somes flank a nucleosome-depleted region (NDR). However, this view has been recently disputed by contradictory reports as to whether wider (greater than or similar to 150 bp) NDRs instead contain unstable, micrococcal nuclease-sensitive ("fragile'') nucleosomal particles. To determine the composition of fragile particles, we introduce CUT&RUN. ChIP, in which targeted nuclease cleavage and release is followed by chromatin immunoprecipitation. We find that fragile particles represent the occupancy of the RSC (remodeling the structure of chromatin) nucleosome remodeling complex and RSC-bound, partially unwrapped nucleosomal intermediates. We also find that general regulatory factors (GRFs) bind to partially unwrapped nucleosomes at these promoters. We propose that RSC binding and its action cause nucleosomes to unravel, facilitate subsequent binding of GRFs, and constitute a dynamic cycle of nucleosome deposition and clearance at the subset of wide Pol II promoter NDRs.