CHARACTERIZATION AND USE OF THE DROSOPHILA METALLOTHIONEIN PROMOTER IN CULTURED DROSOPHILA-MELANOGASTER CELLS
CHARACTERIZATION AND USE OF THE DROSOPHILA METALLOTHIONEIN PROMOTER IN CULTURED DROSOPHILA-MELANOGASTER CELLS
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DOI：
10.1093/nar/16.3.1043
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发表时间：
1988-02-11
影响因子：
14.9
通讯作者：
GOLDSTEIN, LSB
中科院分区：
文献类型：
--
作者：
BUNCH, TA;GRINBLAT, Y;GOLDSTEIN, LSB
来自金属硫蛋白基因的启动子可能是用于构建待在培养的果蝇细胞中表达的嵌合基因的有用的条件启动子。为了探索这种可能性，内源性金属硫蛋白基因和在体外构建的嵌合基因含有金属硫蛋白启动子的反应进行了检查。当添加到果蝇Schneider's 2系细胞的生长培养基中时，铜和镉可以产生30-100倍的金属硫蛋白mRNA水平的诱导。诱导的水平取决于添加到培养基中的铜或镉的量，并且这些mRNA水平保持高水平至少四天。铜的毒性比镉小，不会在细胞中引起典型的热休克反应。最后，含有金属硫蛋白启动子的嵌合基因在转化到细胞中时显示出类似的诱导作用。
The promoter from the metallothionein gene may be a useful conditional promoter for the construction of chimeric genes to be expressed in Drosophila cells in culture. To explore this possibility the responses of the endogenous metallothionein gene and an in vitro constructed chimeric gene containing the metallothionein promoter were examined. Copper and cadmium, when added to the growth medium of Drosophila Schneider''s line 2 cells, can produce a 30-100 fold induction of metallothionein mRNA levels. The level of induction depends on the amount of copper or cadmium added to the medium and these mRNA levels remain high for at least four days. Copper is less toxic than cadmium and does not induce a typical heat-shock response in the cells. Finally, a chimeric gene containing the metallothionein promoter shows a similar induction when transformed into the cells.