A transgenic Lef1/β-catenin-dependent reporter is expressed in spatially restricted domains throughout zebrafish development
A transgenic Lef1/β-catenin-dependent reporter is expressed in spatially restricted domains throughout zebrafish development
复制标题
DOI：
10.1006/dbio.2001.0515
复制
发表时间：
2002-01-15
影响因子：
2.7
通讯作者：
Moon, RT
中科院分区：
文献类型：
--
作者：
Dorsky, RI;Sheldahl, LC;Moon, RT
Wnt/β-连环蛋白信号通路在胚胎发育过程中起着多种作用，其中只有少数已经被广泛表征。虽然在整个胚胎发生过程中已经鉴定了Wnt表达的结构域，但响应细胞的解剖学和分子表征大多未被探索。我们已经产生了一个转基因斑马鱼线，表达不稳定的绿色荧光蛋白（GFP）的控制下的β-连环蛋白响应启动子的变体。该转基因（TOPdGFP）的早期合子表达反映了胚胎中Wnt信号传导的已知结构域。左侧活性的丧失导致报告基因表达降低和后部缺陷，而Tcf 3（无头，Hd 1）活性的丧失不改变报告基因表达，即使它导致前脑结构的丧失。此外，异位Wnt 1表达可以激活报告基因。在较老的胚胎中，我们确定了一些转基因表达细胞群作为β-连环蛋白信号传导的新位点。我们的结论是，我们的TOP-dGFP报告线忠实地说明了β-连环蛋白活性的结构域，并能够识别响应细胞群。(C)2001年，爱思唯尔科学。
The Wnt/beta-catenin signaling pathway plays multiple roles during embryonic development, only a few of which have been extensively characterized. Although domains of Wnt expression have been identified throughout embryogenesis, anatomical and molecular characterization of responding cells has been mostly unexplored. We have generated a transgenic zebrafish line that expresses a destabilized green fluorescent protein (GFP) variant under the control of a beta-catenin responsive promoter. Early zygotic expression of this transgene (TOPdGFP) mirrors known domains of Wnt signaling in the embryo. Loss of Left activity results in decreased reporter expression and posterior defects, while loss of Tcf3 (Headless, Hd1) activity does not alter reporter expression, even though it results in loss of forebrain structures. In addition, ectopic Wnt1 expression can activate the reporter. In older embryos, we identify a number of transgene-expressing cell populations as novel sites of beta-catenin signaling. We conclude that our TOP-dGFP reporter line faithfully illustrates domains of beta-catenin activity and enables the identification of responsive cell populations. (C) 2001 Elsevier Science.