IgG-mediated systemic anaphylaxis to protein antigen can be induced even under conditions of limited amounts of antibody and antigen
IgG-mediated systemic anaphylaxis to protein antigen can be induced even under conditions of limited amounts of antibody and antigen
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DOI：
10.1016/j.bbrc.2010.10.098
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发表时间：
2010-11-26
影响因子：
3.1
通讯作者：
Karasuyama, Hajime
中科院分区：
文献类型：
--
作者：
Ishikawa, Ryosuke;Tsujimura, Yusuke;Karasuyama, Hajime
全身性过敏反应是一种急性严重且可能致命的过敏反应。两类抗体IgE和IgG通过不同的机制与效应细胞和化学介质的不同使用促成小鼠中过敏反应的发展。据报道，诱导IgG介导的过敏反应需要比IgE更大量的抗体和抗原。介导的一个，表明前者可能不经常发生，因为后者在真实的生活中为了重新解决这个问题，我们在本研究中建立了一个新的小鼠模型，被动IgG介导的全身过敏反应，以天然蛋白抗原卵清蛋白（OVA）而不是先前研究中使用的人工半抗原化的蛋白抗原。用特异于不同OVA表位的混合物（但不是单个IgG 1 mAb）对小鼠进行被动致敏，引起响应于OVA攻击的全身过敏反应。重要的是，比先前报道的小得多的抗体和抗原剂量足以诱导IgG-1 mAb。介导的全身过敏反应此外，当小鼠被动致敏抗原时，相对小剂量的抗原可通过IgE介导的机制和IgG介导的机制诱导严重的过敏反应，特异性IgE和IgG这些结果强烈表明IgG介导的全身过敏反应在抗体介导的全身过敏反应中并不罕见，与以前的想法相反，并且至少在小鼠中显著有助于真实的生活中的主动全身过敏反应（C）2010 Elsevier Inc保留所有权利
Systemic anaphylaxis is an acute severe and potentially fatal allergic reaction Two classes of antibodies IgE and IgG contribute to the development of anaphylaxis in mice through different mechanisms with distinct usage of effector cells and chemical mediators Larger quantities of antibody and antigen are reportedly required to induce IgG-mediated anaphylaxis than IgE-mediated one suggesting that the former may not happen as frequently as the latter in real life To readdress this Issue we established in the present study a novel mouse model of passive IgG-mediated systemic anaphylaxis to a native protein antigen ovalbumin (OVA) rather than artificially haptenated protein antigens used in previous studies Passive sensitization of mice with a cocktail of but not individual IgG1 mAbs specific to distinct OVA epitopes elicited systemic anaphylaxis in response to OVA challenge Importantly much smaller doses of antibody and antigen than previously reported were sufficient for the induction of IgG-mediated systemic anaphylaxis Moreover a relatively small dose of antigen could induce severe anaphylaxis through both IgE- and IgG-mediated mechanisms when mice had been passively sensitized with antigen-specific IgE and IgG These results strongly suggest that IgG-mediated systemic anaphylaxis is not rare among antibody-mediated systemic anaphylaxis in contrast to previous thought and significantly contributes to active systemic anaphylaxis in real life at least in mice (C) 2010 Elsevier Inc All rights reserved