Growth and Osteogenic Differentiation of Adipose Stem Cells on PLA/Bioactive Glass and PLA/β-TCP Scaffolds
Growth and Osteogenic Differentiation of Adipose Stem Cells on PLA/Bioactive Glass and PLA/β-TCP Scaffolds
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DOI：
10.1089/ten.tea.2008.0241
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发表时间：
2009-07-01
影响因子：
4.1
通讯作者：
Suuronen, Riitta
中科院分区：
文献类型：
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作者：
Haimi, Suvi;Suuriniemi, Niina;Suuronen, Riitta
本研究的目的是比较由生物活性相(生物活性玻璃或β-磷酸三钙[β-磷酸三钙]10和20wt%)组成的新型三维复合支架对人脂肪干细胞(ASC)活性、分布、增殖和成骨分化的影响。用荧光染色、环境扫描电子显微镜和扫描电子显微镜检测ASCs在生物活性复合支架上的活性和分布。生物活性玻璃和β-磷酸三钙在聚乳酸支架中对ASCs的增殖和成骨分化无明显影响。培养2周后，以聚乳酸/β-磷酸三钙复合支架培养的ASCs的DNA含量和碱性磷酸酶(ALP)活性均高于其他支架类型。有趣的是，聚乳酸/生物活性玻璃支架的ASCs细胞数显著低于其他三种支架，但其ALP/DNA比值显著高于其他三种支架。这些结果表明，与其他类型的支架相比，聚乳酸/β-磷酸三钙复合支架显著促进了ASC的增殖和总碱性磷酸酶的活性。这支持了聚乳酸/β-磷酸三钙复合材料作为有效的组织工程支架和骨替代材料的潜在应用前景。
The aim of this study was to compare the effects of novel three-dimensional composite scaffolds consisting of a bioactive phase (bioactive glass or beta-tricalcium phosphate [beta-TCP] 10 and 20 wt%) incorporated within a polylactic acid (PLA) matrix on viability, distribution, proliferation, and osteogenic differentiation of human adipose stem cells (ASCs). The viability and distribution of ASCs on the bioactive composite scaffolds was evaluated using Live/Dead fluorescence staining, environmental scanning electron microscopy, and scanning electron microscopy. There were no differences between the two concentrations of bioactive glass and beta-TCP in PLA scaffolds on proliferation and osteogenic differentiation of ASCs. After 2 weeks of culture, DNA content and alkaline phosphatase (ALP) activity of ASCs cultured on PLA/beta-TCP composite scaffolds were higher relative to other scaffold types. Interestingly, the cell number was significantly lower, but the relative ALP/DNA ratio of ASCs was significantly higher in PLA/bioactive glass scaffolds than in other three scaffold types. These results indicate that the PLA/beta-TCP composite scaffolds significantly enhance ASC proliferation and total ALP activity compared to other scaffold types. This supports the potential future use of PLA/beta-TCP composites as effective scaffolds for tissue engineering and as bone replacement materials.