Fast exhaustive enrichment and electrochemical quantitative detection of anthocyanins from natural products by using dual responsive and dummy molecularly imprinted polymers
Fast exhaustive enrichment and electrochemical quantitative detection of anthocyanins from natural products by using dual responsive and dummy molecularly imprinted polymers
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DOI：
10.1016/j.microc.2022.107545
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发表时间：
2022-04
影响因子：
4.8
通讯作者：
Zhuang-Fei Jiang;Qin Li;Qing-Yao Li;Hui Xu;Jia-Yuan He;Chong-Zhi Wang;Lian-di Zhou;Qihui Zhang-Qihu
中科院分区：
文献类型：
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作者：
Zhuang-Fei Jiang;Qin Li;Qing-Yao Li;Hui Xu;Jia-Yuan He;Chong-Zhi Wang;Lian-di Zhou;Qihui Zhang-Qihu
本研究利用虚拟模板分子印迹技术、磁分离技术和pH响应技术合成了一种新型吸附剂，用于水果中花色苷的快速富集和电化学检测。以槲皮素为虚拟模板，4-乙烯基苯硼酸和甲基丙烯酸为功能单体，在磁性微粒表面合成了分子印迹材料。采用扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、N2吸附-脱附、热重分析、振动样品磁强计和X射线光电子能谱等手段对样品的微观结构进行了表征。与结构类似物相比，虚拟模板磁性pH响应分子印迹聚合物（DMMIPs）具有更短的动力学平衡时间、更高的选择性和更好的对花色苷的吸附能力。模拟分子印迹聚合物的结合容量和印迹因子分别达到59.22mg/g和1.56。蓝莓和葡萄皮粗提物中花色苷的含量与文献报道一致。
In this work, a new adsorbent was synthesized using dummy-template molecular imprinting technology, magnetic separation technology and pH-responsive technology, which was used to quickly enrich anthocyanins in fruit and detect with electrochemical methods. On the surface of magnetic particles, the molecularly-imprinted materials were synthesized by using quercetin as the dummy-template, 4-vinyl phenylboronic acid and methacrylic acid as the functional monomers. The microstructure was characterized by scanning electron microscopy, transmission electron microscope, Fourier transform infrared spectroscopy, N2adsorption–desorption, thermal gravimetric analysis, vibrating sample magnetometer and X-ray photoelectron spectroscopy. Compared with structural analogue, dummy-template magnetic and pH-responsive molecularly imprinted polymers (DMMIPs) had shorter kinetic equilibrium time, higher selectivity and better adsorption capacity toward anthocyanins. The binding capacity and imprinting factor of the dummy molecularly imprinted polymer could reach 59.22 mg/g and 1.56, respectively. The anthocyanins’ content of blueberry and grape peel crude extracts were consistent with the reference reports.