Cloning of SEZ-12 encoding seizure-related and membrane-bound adhesion protein
Cloning of SEZ-12 encoding seizure-related and membrane-bound adhesion protein
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DOI：
10.1006/bbrc.1996.0712
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发表时间：
1996-05-06
影响因子：
3.1
通讯作者：
Sugaya, E
中科院分区：
文献类型：
--
作者：
Kajiwara, K;Nagasawa, H;Sugaya, E
SEZ-12是从小鼠大脑皮层原代培养的神经元中分离到的与癫痫相关的cdna之一，该细胞含有或不含戊四唑（PTZ）。注射PTZ可使小鼠脑内SEZ-12的表达瞬间下调。为了鉴定SEZ-12，我们进行了全长cDNA的分离和核苷酸序列分析。SEZ-12的氨基酸序列分析表明，SEZ-12编码膜结合c型凝集素，与从DiGeorge综合征相关的平衡易位断点克隆的人类cDNA、DGCR2和IDD具有显著的同源性。分离得到的cDNA长度约为4kb，该信息在低丰度的各种器官中普遍表达。在此之前，我们还利用差分杂交技术克隆了一个可能参与细胞间相互作用的跨膜蛋白。这些发现表明，神经元细胞的跨膜信号传导可能在ptz诱导的癫痫发作中起重要作用。(C) 1996学术出版社，Inc.
SEZ-12 is one of the seizure-related cDNAs which was isolated by differential hybridization from primary cultured neurons from the mouse cerebral cortex with or without pentylenetetrazol (PTZ). SEZ-12 expression is transiently down-regulated in the mouse brain by injection of PTZ. To characterize SEZ-12, isolation of full-length cDNA and nucleotide sequence analysis were performed. The deduced amino acid sequence of SEZ-12 revealed that it encodes membrane-bound C-type lectin and has a significant homology to that of human cDNA, DGCR2 and IDD, which were cloned from a balanced translocation breakpoint associated with the DiGeorge syndrome. The isolated cDNA was about 4 kb in length and the message was expressed ubiquitously in various organs with low-abundance. Previously, we also cloned a transmembrane protein which is probably involved in cell-cell interaction by the differential hybridization technique. These findings suggest that transmembrane signaling in neuronal cells may have an important role in PTZ-induced seizure. (C) 1996 Academic Press, Inc.