Endothelialized microvasculature based on a biodegradable elastomer
Endothelialized microvasculature based on a biodegradable elastomer
复制标题
DOI：
10.1089/ten.2005.11.302
复制
发表时间：
2005-01-01
影响因子：
--
通讯作者：
Wang, YD
中科院分区：
文献类型：
--
作者：
Fidkowski, C;Kaazempur-Mofrad, MR;Wang, YD
重要器官维持致密的微血管系统以维持其细胞的正常功能。为了使组织工程器官正常工作，必须开发人工毛细血管网络。我们有一个可生物降解和生物相容性的弹性体，聚（甘油癸二酸酯）（PGS）的微加工毛细血管网络。我们通过标准的微机电系统（MEMS）技术将毛细管图案蚀刻到硅晶片上。所得的硅晶片用作器件的微模具。我们将图案化的PGS膜与平膜粘合在一起，以创建毛细血管网络，并用注射泵以生理流速灌注。该装置在流动条件下内皮化，并且部分管腔在培养的14天内达到汇合。这种方法可能导致组织工程微血管，这是至关重要的器官工程。
Vital organs maintain dense microvasculature to sustain the proper function of their cells. For tissue-engineered organs to function properly, artificial capillary networks must be developed. We have microfabricated capillary networks with a biodegradable and biocompatible elastomer, poly( glycerol sebacate) ( PGS). We etched capillary patterns onto silicon wafers by standard microelectromechanical systems (MEMS) techniques. The resultant silicon wafers served as micromolds for the devices. We bond the patterned PGS film with a flat film to create capillary networks that were perfused with a syringe pump at a physiological flow rate. The devices were endothelialized under flow conditions, and part of the lumens reached confluence within 14 days of culture. This approach may lead to tissue-engineered microvasculature that is critical in vital organs engineering.