Short-term response of arbuscular mycorrhizal association to spider mite herbivory
Short-term response of arbuscular mycorrhizal association to spider mite herbivory
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DOI：
10.1007/s10144-008-0116-2
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发表时间：
2009-04
影响因子：
1.7
通讯作者：
T. Nishida;Naoyuki Izumi;N. Katayama;T. Ohgushi
中科院分区：
文献类型：
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作者：
T. Nishida;Naoyuki Izumi;N. Katayama;T. Ohgushi
研究了荨麻叶螨（Tetranychus urticae）对盆栽植物丛枝菌根真菌（AMF;Gigaspora margarita）定殖和活性的影响。我们通过两种方法评估了丛枝菌根（AM）关联的变化：(1)常规的菌根菌丝台锥蓝染色来检测根中的AMF生物量（菌根定植）；(2)菌根酶（琥珀酸脱氢酶，SDH）的重要染色来检测菌根活性（SDH活性）。地上植草后4 d菌根定植量和SDH活性开始增加，7 d和12 d分别恢复到不植螨时的初始水平。这些结果表明，螨虫草食反应中AM缔合的变化是一种短期反应。
We examined effects of aboveground herbivory by spider mites (Tetranychus urticae) on colonization and activity of arbuscular mycorrhizal fungi (AMF;Gigaspora margarita) using potted plants (Lotus japonicus). We evaluated changes in arbuscular mycorrhizal (AM) association two ways: (1) conventional trypan blue staining of mycorrhizal hyphae to examine AMF biomass in roots (mycorrhizal colonization) and (2) vital staining for a mycorrhizal enzyme (succinate dehydrogenase, SDH) to examine mycorrhizal activity (SDH activity). Mycorrhizal colonization and SDH activity started to increase 4 days after aboveground herbivory, and returned to the initial levels in the absence of mite herbivory in 7 and 12 days, respectively. These results suggest that the change in AM association in response to mite herbivory is a short-term response.