Optical pH measurement system using a single fluorescent dye for assessing susceptibility to dental caries
Optical pH measurement system using a single fluorescent dye for assessing susceptibility to dental caries
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DOI：
10.1117/1.jbo.24.1.017001
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发表时间：
2019-01
影响因子：
3.5
通讯作者：
Manuja Sharma;Jasmine Y. Graham;Philip A Walczak;R. Nguyen;Lauren K. Lee;Matthew D. Carson;Leonard Y. Nelson;Shwetak N. Patel;Zheng Xu;E. Seibel
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文献类型：
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作者：
Manuja Sharma;Jasmine Y. Graham;Philip A Walczak;R. Nguyen;Lauren K. Lee;Matthew D. Carson;Leonard Y. Nelson;Shwetak N. Patel;Zheng Xu;E. Seibel
抽象。高糖饮食和不良的口腔卫生促进生物膜（牙菌斑）的形成，该生物膜牢固地粘附在牙釉质表面，并促进耐酸细菌的进化。酸有助于外部牙釉质的脱矿，其在pH值下降到临界值（0.55）以下持续较长时间后加速，从而导致需要进行修复程序。提醒牙医和龋齿易感患者注意龋齿易感性的预防性技术需要牙菌斑活性的临床测量。因此，有必要评估牙菌斑沉积物的产酸能力的窝沟和缝隙的咬合和邻间区域。已经使用FDA批准的染料和LED激发开发了比率荧光pH传感装置。使用光谱仪收集黄绿素光谱分布，并用光谱解混算法分析，用于在4.5至7的pH范围内校准。使用蔗糖冲洗，以加速细菌代谢，并测量pH值随时间的下降在人体受试者的体内试验研究。光学系统是相对免疫的混杂因素，如光漂白，染料浓度，和变化的激发强度与早期染料为基础的pH值测量技术。
Abstract. Sugar-rich diets and poor dental hygiene promote the formation of a biofilm (plaque) that strongly adheres to the dental enamel surface and fosters the evolution of aciduric bacteria. The acid contributes to demineralization of the exterior tooth enamel, which accelerates after the pH drops below a critical value (∼5.5) for extended time periods resulting in the need for restorative procedures. Preventative techniques to alert the dentist and caries-susceptible patients regarding vulnerability to dental decay require a clinical measure of plaque activity. Therefore, there is a need to evaluate the acid production capability of plaque deposits in the pits and fissures of occlusal and interproximal regions. A ratiometric fluorescence pH-sensing device has been developed using an FDA-approved dye and LED excitation. Fluorescein spectral profiles were collected using a spectrometer and analyzed with a spectral unmixing algorithm for calibration over the pH range of 4.5 to 7. An in vivo pilot study on human subjects was performed using a sucrose rinse to accelerate bacterial metabolism and to measure the time-dependent drop in pH. The optical system is relatively immune to confounding factors such as photobleaching, dye concentration, and variation in excitation intensity associated with earlier dye-based pH measurement techniques.