Human milk oligosaccharides are minimally digested in vitro
Human milk oligosaccharides are minimally digested in vitro
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DOI：
10.1093/jn/130.12.3014
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发表时间：
2000-12-01
影响因子：
4.2
通讯作者：
Rudloff, S
中科院分区：
文献类型：
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作者：
Gnoth, MJ;Kunz, C;Rudloff, S
在检查母乳低聚糖（HMO）的功能方面，尚不清楚它们是否在通过婴儿胃肠道的过程中被消化。HMO从单独的牛奶样品（n = 6）制备，并通过色谱法分离成中性和酸性化合物。这些低聚糖组分的消化率进行了研究，由人唾液淀粉酶，猪胰腺淀粉酶和刷状缘膜囊泡（BBMV）从猪小肠分离，我们还研究了这些结构的低pH值的影响。HMO及其消化产物的表征通过高pH阴离子交换色谱-脉冲安培检测（HPAEC-PAD）以及TLC进行。结果表明，唾液淀粉酶和胰淀粉酶都不裂解HMO。仅在与BBMV孵育2小时后，观察到HMO的轻微改变。HPAEC-PAD分析揭示了中性低聚糖组分中的两种新组分;这些组分通过质谱分析表征为乳-N-丙糖和半乳糖。只有乳糖-N-丙糖存在于低聚糖的消化测定中，低聚糖不含岩藻糖基或N-乙酰神经氨酸残基。这些结果表明
In examining the functional aspects of human milk oligosaccharides (HMO), it is not known whether they are digested during the passage through the infant's gastrointestinal tract. HMO were prepared from individual milk samples (n = 6) and separated into neutral and acidic compounds by chromatography. These oligosaccharide fractions were studied for their digestibility by human salivary amylase, porcine pancreatic amylase and brush border membrane vesicles (BBMV) isolated from porcine small intestine; we also examined the effect of low pH on these structures. The characterization of HMO and their digestion products was performed by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) as well as TLC. It was shown that neither salivary amylase nor pancreatic amylase cleaved HMO. Only after a 2-h incubation with BBMV were slight modifications of the HMO observed. HPAEC-PAD analysis revealed two new components within the neutral oligosaccharide fractions; these were characterized by mass spectrometric analysis as lacto-N-triose and galactose. Only lacto-N-triose was present within digestion assays of oligosaccharides, which did not contain fucosyl or N-acetylneuraminic acid residues. These results suggest that