Leaf width gene LW5/D1 affects plant architecture and yield in rice by regulating nitrogen utilization efficiency
Leaf width gene LW5/D1 affects plant architecture and yield in rice by regulating nitrogen utilization efficiency
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叶宽基因LW5/D1通过调节氮素利用效率影响水稻株型和产量
DOI：
10.1016/j.plaphy.2020.10.035
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发表时间：
2020-12-01
影响因子：
6.5
通讯作者：
Zhang, Guangheng
中科院分区：
文献类型：
--
作者：
Zhu, Yuchen;Li, Ting;Zhang, Guangheng
叶子是负责光合作用的主要结构，使叶子形态成为水稻植物建筑的最重要特征之一。植物结构和营养利用都共同影响了水稻产量，但是，它们的分子关联仍然很少了解。我们确定了一个水稻突变体，叶宽5（LW5），该叶子显示出小谷物和宽叶，并具有典型的小“水槽”和大“源”的特征。基于地图的克隆和CRISPR-CAS9基因编辑表明LW5既影响植物的建筑和产量。它是D1的等位基因，编码米G蛋白A亚基。 LW5功能的损失导致光合作用率，血管束和叶绿素含量的增加。然而，晶粒晶格的比率和谷物填充速率显着降低。 15 N-硝酸盐的检测结果和与氮相关的基因的表达分析表明，LW5在硝酸盐摄取和运输中起重要作用。 LW5通过调节氮的转移来影响植物的建筑和晶粒尺寸。这些结果为围绕水稻中“源 - 平衡”平衡的分子机制进行进一步研究提供了理论基础，并提出了用于在理想植物类型中种植超级稻的分子设计的新型方法。
Leaves are the primary structures responsible for photosynthesis, making leaf morphology one of the most important traits of rice plant architecture. Both plant architecture and nutrient utilization jointly affect rice yield, however, their molecular association is still poorly understood. We identified a rice mutant, leaf width 5 (lw5), that displayed small grains and wide leaves and possesses characteristics typical of a small "sink" and a large "source". Map-based cloning and CRISPR-Cas9 gene editing indicated that LW5 affects both the plant architecture and yield. It is an allele of D1, encoding the rice G protein a subunit. The loss of LW5 functioning leads to an increase in the rate of photosynthesis, vascular bundles, and chlorophyll content. However, the grain-straw ratio and the rate of grain filling decreased significantly. The detection results of 15 N-ammonium nitrate and an expression analysis of genes associated with nitrogen demonstrated that LW5 serves an important role in nitrate uptake and transport. LW5 affects plant architecture and grain size by regulating nitrogen transfer. These results provide a theoretical foundation for further research surrounding the molecular mechanism of "source-sink" balance in rice and suggest novel methods of molecular design for the cultivation of breeding super rice in ideal plant types.