The antiproliferative effects of PPARgamma ligands in normal human mammary epithelial cells.
The antiproliferative effects of PPARgamma ligands in normal human mammary epithelial cells.
复制标题
PPARgamma 配体在正常人乳腺上皮细胞中的抗增殖作用。
DOI：
10.1023/a:1022978608125
复制
发表时间：
2003
影响因子：
3.8
通讯作者：
Seewaldt,VictoriaL
中科院分区：
文献类型：
--
作者：
Yee,LisaD;Guo,Yan;Bradbury,Jamie;Suster,Saul;Clinton,StevenK;Seewaldt,VictoriaL
Peroxisome proliferator-activated receptor γ (PPARγ) is a transcription factor in the steroid nuclear receptor superfamily. Ligand activation of PPARγ is associated with differentiation and an inhibition of proliferation in normal and malignant cells, including adipocytes, monocytes, and tumor cells in colon, prostate, and breast cancers. The current studies were undertaken to assess both the expression and functional activity of PPARγ in cultured normal human mammary epithelial cells (HMECs) and tissue samples. Analyses by northern hybridization, immunoblotting, and immunohistochemistry demonstrate PPARγ gene expression in HMECs and breast tissue specimens. DNA binding and transactivation assays indicate the presence of functionally active PPARγ in HMECs. Treatment with PPARγ selective ligands, 15-deoxy-Δ-12, 14-prostaglandin J2 (15dPGJ2) and ciglitazone, inhibits the growth of HMECs in a dose-dependent manner. This growth inhibition is associated with alterations in cell cycle progression and the induction of apoptosis.
