Comparison of Gingiva, Dental Pulp, and Periodontal Ligament Cells From the Standpoint of Mesenchymal Stem Cell Properties
Comparison of Gingiva, Dental Pulp, and Periodontal Ligament Cells From the Standpoint of Mesenchymal Stem Cell Properties
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DOI：
10.3727/215517912x653319
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发表时间：
2012-04-01
期刊：
影响因子：
--
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
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作者：
Otabe, Koji;Muneta, Takeshi;Sekiya, Ichiro
口腔组织中的间充质干细胞（MSC）的具体特性仍然未知，尽管之前已经报道过它们的存在。我们从拔除的牙齿中收集了牙龈、牙髓和牙周膜组织并分离了 MSC。通过患者匹配分析，对这些 MSC 的每颗牙齿产量、表面表位和分化潜力进行比较。为了进行体内钙化分析，将安装在β-磷酸三钙TCP上的大鼠牙龈和牙髓细胞移植到大鼠的椎周肌肉中6周。每颗牙齿的牙龈细胞和牙髓细胞的产量高于牙周膜细胞（n = 6，p < 0.05）。牙周膜细胞的产量太低，无法进行进一步分析。牙龈和牙髓细胞表达 MSC 标记物，例如 CD44、CD90 和 CD166。牙龈和牙髓细胞在体外获得软骨细胞和脂肪细胞的表型。体外大约60%的牙龈细胞集落和40%的牙髓细胞集落被茜素红染色呈阳性，体内牙龈和牙髓细胞均钙化。我们阐明了源自拔除牙齿的 MSC 的特性。我们可以从牙龈和牙髓中获得高产量的具有成骨潜力的间充质干细胞。这些结果表明牙龈和牙髓是硬组织再生的推定细胞来源。
The specific properties of mesenchymal stem cells (MSCs) in oral tissues still remain unknown though their existence has been previously reported. We collected gingiva, dental pulp, and periodontal ligament tissues from removed teeth and isolated MSCs. These MSCs were compared in terms of their yields per tooth, surface epitopes, and differentiation potentials by patient-matched analysis. For in vivo calcification analysis, rat gingival and dental pulp cells mounted on beta-tricalcium phospateTCP were transplanted into the perivertebral muscle of rats for 6 weeks. Gingival cells and dental pulp cells showed higher yield per tooth than periodontal ligament cells (n = 6, p < 0.05). Yields of periodontal ligament cells were too low for further analysis. Gingival and dental pulp cells expressed MSC markers such as CD44, CD90, and CD166. Gingival and dental pulp cells obtained phenotypes of chondrocytes and adipocytes in vitro. Approximately 60% of the colonies of gingival cells and 40% of the colonies of dental pulp cells were positively stained with alizarin red in vitro, and both gingival and dental pulp cells were calcified in vivo. We clarified properties of MSCs derived from removed teeth. We could obtain a high yield of MSCs with osteogenic potential from gingiva and dental pulp. These results indicate that gingiva and dental pulp are putative cell sources for hard tissue regeneration.