Profiling of differentially expressed genes in LRRC4 overexpressed glioblastoma cells by cDNA array.
Profiling of differentially expressed genes in LRRC4 overexpressed glioblastoma cells by cDNA array.
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DOI：
10.1111/j.1745-7270.2005.00100.x
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发表时间：
2005-10
影响因子：
3.7
通讯作者：
Qiu-hong Zhang;Ming‐hua Wu;Li-li Wang;Li Cao;Ke Tang;C. Peng;Kai Gan;Xiao-Ling Li;Gui-yuan Li-Gui
中科院分区：
文献类型：
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作者：
Qiu-hong Zhang;Ming‐hua Wu;Li-li Wang;Li Cao;Ke Tang;C. Peng;Kai Gan;Xiao-Ling Li;Gui-yuan Li-Gui
我们之前的研究表明，LRRC4是富含亮氨酸重复序列（LRR）超家族的新成员，具有抑制脑肿瘤生长的潜力。为了进一步分析LRRC4在维持中枢神经系统正常功能和抑制肿瘤发生方面的功能，我们利用Atlas阵列研究了两个可诱导双稳定过表达LRRC4的细胞系中神经生物学相关基因的表达变化。Atlas膜上发现的588个基因中，有17个在LRRC4转染的U251MG Tet-on细胞中表达水平发生改变，这些基因参与细胞增殖和细胞周期进程、肿瘤侵袭和转移以及神经递质合成和释放。此外，细胞侵袭实验结果显示，LRRC4可以抑制U251MG细胞的迁移。这些研究首次通过cDNA阵列分析了LRRC4对不同神经生物学基因参与U251MG胶质母细胞瘤细胞的影响，并为LRRC4在胶质瘤中的功能提供了新的见解。
Our previous study has shown that LRRC4 is a novel member of the leucine-rich repeat (LRR) superfamily and has the potential to suppress brain tumor growth. In order to further analyze the functions of LRRC4 on the maintenance of normal function and suppression of tumorigenesis in the central nervous system, we investigated alterations in gene expression related to neurobiology by the Atlas array in two inducible dual-stable LRRC4-overexpressing cell lines. Seventeen of 588 genes spotted on the Atlas membrane showed altered expression levels in LRRC4 transfected U251MG Tet-on cells, which are involved in cell proliferation and cell cycle progression, tumor invasion and metastasis, and neurotransmitter synthesis and release. In addition, cell invasion assay results showed that LRRC4 can inhibit the U251MG cell migration. These studies represent the first cDNA array analysis of the effects of LRRC4 on the involvement of different neurobiological genes in U251MG glioblastoma cells and provide new insights into the function of LRRC4 in glioma.