Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant antibacterial and cytotoxic effects
Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant antibacterial and cytotoxic effects
复制标题
DOI：
10.1002/aoc.4458
复制
发表时间：
2018-09-01
影响因子：
3.9
通讯作者：
Veisi, Hojat
中科院分区：
文献类型：
--
作者：
Hamelian, Mona;Zangeneh, Mohammad Mandi;Veisi, Hojat
本研究采用环境友好、成本低廉、简便易行的方法，以红蓟提取物为还原剂和封端剂，在室温下合成了银纳米粒子。各种分析技术包括UV-Vis吸收光谱法确定溶液中Ag NP的存在，通过FT-IL结晶度证实了在Ag NP的还原和封端过程中Thuringus Kotschyanus提取物的官能团，其中从X射线衍射（XRD）图案证实了fcc平面，能量色散光谱（EDS）确定了样品中元素的存在，表面形态，利用扫描电子显微镜（SEM）、原子力显微镜（AFM）和高分辨透射电子显微镜（HRTEM）显示了不同形状和尺寸的银纳米颗粒。利用热重分析（TGA）测定了银纳米粒子的起始和终止破坏温度。此外，银纳米粒子的抗菌，抗氧化和细胞毒性性能进行了研究。琼脂平板法和琼脂孔扩散法是测定纳米银抗菌性能的方法。此外，通过大量肉汤稀释试验识别MIC（最小抑菌浓度）和MBC（最小杀菌浓度）。DPPH自由基清除试验用于抗氧化性能，并与作为标准抗氧化剂的二丁基羟基甲苯（BHT）进行比较，其显示出比BHT更高的抗氧化活性。合成的银纳米颗粒以剂量依赖性方式具有很大的细胞活力，并证明这种合成银纳米颗粒的方法具有无毒性。合成的Ag纳米颗粒的平均直径为约50-60 nm。
Present study used ecofriendly, cost efficient and easy method for synthesis of silver nanoparticles (Ag NPs) at the room temperature by Thymus Kotschyanus extract as reducing and capping agent. Various analytical technique including UV-Vis absorption spectroscopy determined presence of Ag NPs in the solution, the functional groups of Thymus Kotschyanus extract in the reduction and capping process of Ag NPs are approved by FT-IL crystallinity with the fcc plane approved from the X-ray diffraction (XRD) pattern, energy dispersive spectroscopy (EDS) determined existence of elements in the sample, surface morphology, diverse shapes and size of present Ag NPs were showed by using scanning electron microscopy (SEM), atomic force microscopy (AFM) and high resolution transmission electron microscopy (HRTEM). Beginning and end destroy temperature of present silver nanoparticles were determined by thermal gravimetric spectroscopy (TGA). In addition, antibacterial, antioxidant and cytotoxicity properties of Ag NPs were studied. Agar disk and agar well diffusion are the methods to determined antibacterial properties of synthesized Ag NPs. Also MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were recognized by macro broth dilution assay. DPPH free radical scavenging assay was used for antioxidant property and compare to butylated hydroxytoluene (BHT) as standard antioxidant that showed high antioxidant activity more than BHT. Synthesized Ag NPs have great cell viability in a dose depended manner and demonstrate that this method for synthesis silver nanoparticles provided nontoxic. The average diameter of synthesized Ag NPs was about 50-60 nm.