Sortase A promotes virulence in experimental Staphylococcus lugdunensis endocarditis
Sortase A promotes virulence in experimental Staphylococcus lugdunensis endocarditis
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DOI：
10.1099/mic.0.070292-0
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发表时间：
2013-10-01
期刊：
影响因子：
2.8
通讯作者：
Foster, Timothy J.
中科院分区：
文献类型：
--
作者：
Heilbronner, Simon;Hanses, Frank;Foster, Timothy J.
路邓葡萄球菌是人类的寄生虫，是一种条件致病菌。它能在健康人中引起一种侵袭性的感染性心内膜炎，类似于金黄色葡萄球菌。在这里，我们比较了基因组测序的S。lugdunensis菌株N920143对S.金黄色葡萄球菌在实验性大鼠心内膜炎模型中的作用。N920143引起的病程比S.金黄色葡萄球菌菌株纽曼。然而，植被与S。金黄色葡萄球菌MRSA菌株COL的毒力因子知之甚少。作为遗传操纵细菌的系统的lugdunensis目前是有限的。在这里，我们报告了一种方法，电穿孔的S。lugdunensis与质粒DNA的转化，并证明转化的低效率是由于保守的I型限制性修饰系统的活性。为了简化转化过程，我们构建了一个E.表达N920143的hsdM/hsdS基因的大肠杆菌菌株。从SL 01 B转化S. lugdunensis菌株从克隆复合体1和2中高效分离。在心内膜炎模型中，缺乏分选酶A的N920143的缺失突变体的毒性显著低于野生型。单一表面蛋白Fbl或vWbl缺陷的突变体与野生型没有显着差异，但表现出降低毒力的趋势。
Staphylococcus lugdunensis is a commensal of humans and an opportunistic pathogen. It can cause an aggressive form of infective endocarditis in healthy humans akin to Staphylococcus aureus. Here we compared the virulence of the genome-sequenced S. lugdunensis strain N920143 to S. aureus in an experimental rat endocarditis model. N920143 caused a milder course of disease with lower levels of bacteraemia and smaller endocardial vegetations than S. aureus strain Newman. However, vegetations were comparable to those produced by S. aureus MRSA strain COL. Little is known about virulence factors of S. lugdunensis as systems to manipulate the bacterium genetically are currently limited. Here, we report a method for electroporation of S. lugdunensis with plasmid DNA and demonstrate that the low efficiency of transformation is due to the activity of a conserved type I restriction modification system. To streamline the transformation process, we constructed SL01B, an E. coli strain expressing the hsdM/hsdS genes of N920143. Modified plasmid DNA isolated from SL01B transformed S. lugdunensis strains from clonal complexes 1 and 2 efficiently. A deletion mutant of N920143 lacking sortase A was significantly less virulent than the wild-type in the endocarditis model. Mutants defective in single surface proteins Fbl or vWbl were not significantly different from the wild-type but showed trends towards reduced virulence.