URACIL UPTAKE IN ESCHERICHIA-COLI K-12 - ISOLATION OF URAA MUTANTS AND CLONING OF THE GENE
URACIL UPTAKE IN ESCHERICHIA-COLI K-12 - ISOLATION OF URAA MUTANTS AND CLONING OF THE GENE
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DOI：
10.1128/jb.177.8.2008-2013.1995
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发表时间：
1995-04-01
影响因子：
3.2
通讯作者：
MYGIND, B
中科院分区：
文献类型：
--
作者：
ANDERSEN, PS;FREES, D;MYGIND, B
在低浓度尿嘧啶利用缺陷的突变体已被分离和表征。所讨论的突变（uraA）图谱靠近编码尿嘧啶磷酸核糖基转移酶的upp基因。通过互补分析，已经分离出互补uraA突变的质粒。uraA基因是双顺反子操纵子中的第二个基因，upp是启动子近端基因。测定了该基因的核苷酸序列，该基因编码疏水膜蛋白，计算的M（r）为45，030。在十二烷基硫酸钠-聚丙烯酰胺凝胶中，已在携带uraA质粒的微细胞的膜部分中鉴定出UraA蛋白。
Mutants defective in utilization of uracil at low concentrations have been isolated and characterized. The mutations in question (uraA) map close to the upp gene encoding uracil phosphoribosyltransferase. By complementation analysis, a plasmid that complements the uraA mutation has been isolated. The uraA gene was shown to be the second gene in a bicistronic operon with upp as the promoter proximal gene, The nucleotide sequence of the gene was determined, and the gene encodes a hydrophobic membrane protein with a calculated M(r) of 45,030. The UraA protein has been identified in sodium dodecyl sulfate-polyacrylamide gels in the membrane fraction of minicells harboring the uraA plasmids.