A novel and sensitive method to monitor helminth infections by faecal sampling
A novel and sensitive method to monitor helminth infections by faecal sampling
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DOI：
10.1016/s0001-706x(02)00089-x
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发表时间：
2002-08-01
期刊：
影响因子：
2.7
通讯作者：
Wilson, RA
中科院分区：
文献类型：
--
作者：
Eberl, M;al-Sherbiny, M;Wilson, RA
Kato-Katz技术是通过估计粪便虫卵负荷诊断曼氏血吸虫病的常规方法。为了提高粪便诊断的灵敏度，我们建立并验证了一种新的分离技术，该技术基于相对于粪便材料的更大密度的活寄生虫卵。随后，在埃及的El-Sharkia，用Kato-Katz涂片作为标准，对27名血吸虫病患者进行粪便检查。我们的技术仅检测到低强度感染（< 100个卵/g）。此外，对连续样本的三重Kato-Katz分析仍然遗漏了7.4%的人类患者，而新方法在第二次分析时正确诊断了100%的样本。我们的结论是，在流行地区，许多患者被系统错过了常规诊断。此外，我们的方法的灵敏度允许其用于非人灵长类动物和人类的临床前和临床疫苗试验，其中粪便虫卵计数的可靠估计至关重要。(C)2002 Elsevier Science B. V.保留所有权利。
The Kato-Katz technique is the method routinely used for diagnosing human schistosomiasis mansoni by estimating faecal egg burdens. To improve the sensitivity of faecal diagnosis, we established and validated a novel separation technique based upon the greater density of viable schistosome eggs relative to faecal material. Subsequently, it was used for faecal examination of 27 schistosomiasis patients in El-Sharkia, Egypt, with Kato-Katz smears as criterion standard. Low intensity infections (< 100 eggs/g) were only detected by our technique. Moreover, triple Kato-Katz analysis on consecutive samples still missed 7.4% of all human patients, whereas the new method diagnosed 100% of samples correctly on second analysis. We conclude that in endemic areas many patients are being systematically missed by routine diagnosis. Moreover, the sensitivity of our method allows its use in proposed pre-clinical and clinical vaccine trials in non-human primates and humans, where reliable estimates of faecal egg counts are essential. (C) 2002 Elsevier Science B.V. All rights reserved.