Acid phosphatase activity in mononuclear phagocytes and the U937 cell line: monocyte-derived macrophages express tartrate-resistant acid phosphatase.
Acid phosphatase activity in mononuclear phagocytes and the U937 cell line: monocyte-derived macrophages express tartrate-resistant acid phosphatase.
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DOI：
10.1182/blood.v67.3.729.729
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发表时间：
1986-03
期刊：
影响因子：
20.3
通讯作者：
R. Snipes;Kw Lam;RC Dodd;TK Gray;Michal Cohen
中科院分区：
文献类型：
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作者：
R. Snipes;Kw Lam;RC Dodd;TK Gray;Michal Cohen
抗酒石酸酸性磷酸酶（TRAcP）被用作破骨细胞的标记物，破骨细胞被认为来源于吞噬细胞或吞噬干细胞前体。为了进一步研究单核细胞吞噬细胞与破骨细胞之间的关系，采用三种不同的技术测定了人外周血单核细胞、单核细胞源性巨噬细胞和U937细胞系中酸性磷酸酶（AcP）的活性。我们发现细胞化学和凝胶电泳导致类似的结果，但比色法不一致。正常人外周血单核细胞同时表达酒石酸盐敏感和耐药AcP。在培养中，这些细胞形成多核体，并表达TRAcP活性，该活性被进一步鉴定为与骨组织相关的同工酶。相比之下，凝胶电泳检测U937细胞不表达TRAcP活性。U937细胞和单核细胞都具有干扰AcP比色分析解释的物质。单核细胞源性巨噬细胞中TRAcP的存在进一步支持了吞噬细胞与骨破骨细胞之间的关系。
Tartrate-resistant acid phosphatase (TRAcP) is used as a marker for osteoclasts, which are believed to be derived from phagocytic cells or phagocyte stem cell precursors. To further investigate the relationship between monocytic phagocytes and osteoclasts, acid phosphatase (AcP) activity was measured by three different techniques in human peripheral blood monocytes, monocyte-derived macrophages, and the U937 cell line. We found that cytochemistry and gel electrophoresis led to similar results, but that the colorimetric assay was inconsistent. Normal human peripheral monocytes expressed both tartrate-sensitive and -resistant AcP. In culture these cells formed polykaryons and expressed TRAcP activity that was further identified as an isoenzyme associated with bone tissue. In contrast, the U937 cells did not express TRAcP activity as measured by gel electrophoresis. Both U937 cells and monocytes possess material that interferes with interpretation of the colorimetric assay of AcP. The presence of TRAcP in monocyte-derived macrophages further supports the relationship between phagocytic cells and bone osteoclasts.