MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.
MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.
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DOI：
10.1038/srep40884
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发表时间：
2017-01-18
影响因子：
4.6
通讯作者：
Simões ZL
中科院分区：
文献类型：
--
作者：
Freitas FC;Pires CV;Claudianos C;Cristino AS;Simões ZL
microRNA（miRNAs）是发育过程的关键调节因子，如细胞命运决定和分化。先前的研究表明，蜜蜂胚胎中的Dicer敲除会破坏功能成熟miRNA的加工并损害胚胎模式。在这里，我们研究了蜜蜂胚胎发生中miRNA的表达谱以及高度保守的miR-34- 5 p在参与昆虫分割的基因调节中的作用。共检测到221条miRNAs在蜜蜂胚胎发育过程中表达，其中97条成熟的miRNAs序列为未知序列。有趣的是，我们观察到在不同胚胎阶段的一些miRNA的5-prime和3-prime臂之间的优势转换;然而，大多数miRNA在胚胎发生的所有阶段都呈现一个优势臂。我们对假定的miRNA靶网络和功能通路的全基因组分析表明，miR-34- 5 p是与胚胎模式和发育相关的基因调控相关的最保守和最相关的miRNA之一。此外，我们通过实验验证了miR-34- 5 p直接与pair-rule（偶跳、毛状、fushi-tarazu转录因子1）和细胞骨架（actin 5C）基因3′-非翻译区的调控元件相互作用。我们的研究表明，miR-34- 5 p可能在昆虫早期发育过程中调控配对规则和细胞骨架基因的表达，并控制昆虫的分节。
MicroRNAs (miRNAs) are key regulators of developmental processes, such as cell fate determination and differentiation. Previous studies showed Dicer knockdown in honeybee embryos disrupt the processing of functional mature miRNAs and impairs embryo patterning. Here we investigated the expression profiles of miRNAs in honeybee embryogenesis and the role of the highly conserved miR-34-5p in the regulation of genes involved in insect segmentation. A total of 221 miRNAs were expressed in honey bee embryogenesis among which 97 mature miRNA sequences have not been observed before. Interestingly, we observed a switch in dominance between the 5-prime and 3-prime arm of some miRNAs in different embryonic stages; however, most miRNAs present one dominant arm across all stages of embryogenesis. Our genome-wide analysis of putative miRNA-target networks and functional pathways indicates miR-34-5p is one of the most conserved and connected miRNAs associated with the regulation of genes involved in embryonic patterning and development. In addition, we experimentally validated that miR-34-5p directly interacts to regulatory elements in the 3′-untranslated regions of pair-rule (even-skipped, hairy, fushi-tarazu transcription factor 1) and cytoskeleton (actin5C) genes. Our study suggests that miR-34-5p may regulate the expression of pair-rule and cytoskeleton genes during early development and control insect segmentation.