Effects of lipoxygenase on the corm formation and enlargement in Gladiolus hybridus
Effects of lipoxygenase on the corm formation and enlargement in Gladiolus hybridus
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DOI：
10.1016/j.scienta.2008.05.019
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发表时间：
2008-09-02
影响因子：
4.3
通讯作者：
Yi, Ming-fang
中科院分区：
文献类型：
--
作者：
He, Xiu-li;Shi, Li-wei;Yi, Ming-fang
为探讨脂氧合酶（LOX 1）对唐菖蒲球茎形成和膨大的影响，以“玫瑰至尊”和“高级红”为材料，通过田间和离体试验，测定了唐菖蒲球茎的生长发育过程、LOX 1活性、内源茉莉酸甲酯（MJ）、蔗糖、淀粉和纤维素含量、LOX同工酶及其基因的表达。田间调查结果表明，LOX 1活性和MJ含量在匍匐茎中最高，而在叶片中未检测到，且‘RS’高于‘AR’。在唐菖蒲球茎的生长发育过程中，球茎和子球中的蔗糖、淀粉和纤维素含量都有不同程度的增加。离体培养结果表明，水杨羟肟酸（SHAM）处理明显延迟了球茎的形成。鲜重、直径和球茎形成率下降，可溶性蛋白、MJ和碳水化合物含量降低，LOX 1活性降低。LOX同工酶活性减弱，两条酶带消失。而MJ处理则提高了这些生理生化指标。RT-PCR结果显示，LOX 1基因的表达在SHAM处理下受到抑制，在MJ处理下受到诱导。这些结果表明，LOX 1可能通过影响茉莉酸的生物合成周期，进而影响糖的积累，从而调控唐菖蒲球茎的生长发育。MJ能促进唐菖蒲球茎的形成，而SHAM则抑制其形成，其中MJ 0.5mmol/L是促进唐菖蒲球茎形成和膨大的最佳浓度。(c)2008 Elsevier B. V.保留所有权利。
To investigate the effects of lipoxygenase (LOX1) on the corm formation and enlargement in Gladiolus hybridus, the field and in vitro researches were conducted to detect the growth and development course, the LOX1 activity, the contents of endogenetic methyl jasmonate (MJ), sucrose, starch and cellulose, as well as LOX isozymes and the expression of its genes in 'Rose Supreme' and 'Advanced Red'. The field investigation showed that the highest activities of LOX1 activity and MJ content in stolons, but not detected in leaves, which were higher in 'RS' than in 'AR'. And the contents of sucrose, starch and cellulose in corm and cormels showed different increase during growth and development of Gladiolus corm. The in vitro investigation showed that the salicylhydroxamic acid (SHAM) treatment caused obviously delayed corm formation. decrease of fresh weight and diameter, and percentage of corm formation; reduced contents of total dissoluble protein, MJ and carbohydrates, and LOX1 activity. Additionally the activity of LOX isozymes became weaker, with two isozyme bands disappeared. In contrast, these physiological and biochemical indexes were increased under MJ treatment. RT-PCR also demonstrated that the expression of LOX1 gene was inhibited under SHAM treatment, induced under MJ treatment. These results suggested that LOX1 might regulate the growth and development of Gladiolus corm through affecting JAs biosynthesis cycles, and then resulting in carbohydrate accumulation. The corm formation could be improved by MJ, but suppressed by SHAM, in which, MJ 0.5 mmol/L was the optimum concentration for promoting corm formation and enlargement in Gladiolus. (c) 2008 Elsevier B.V. All rights reserved.