Chromosome painting shows that the proboscis monkey (Nasalis larvatus) has a derived karyotype and is phylogenetically nested within Asian colobines
Chromosome painting shows that the proboscis monkey (Nasalis larvatus) has a derived karyotype and is phylogenetically nested within Asian colobines
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DOI：
10.1002/ajp.10095
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发表时间：
2003-07-01
影响因子：
2.4
通讯作者：
Schempp, W
中科院分区：
文献类型：
--
作者：
Bigoni, F;Stanyon, R;Schempp, W
长鼻猴（Nasalis laruatus）的特殊二倍体数量（2n = 48）在将长鼻猴视为最原始的colobine和长期孤立的群属中发挥了关键作用。在这份报告中，我们使用分子细胞遗传学方法来绘制长鼻猴的染色体同源性，以验证这些假设。结果表明，N. laruatus核型是衍生的，并且相对于其他colobines（2n = 44）和大多数其他旧大陆猴不是原始的。2n = 48的二倍体数目可以通过人类染色体14和6的一段衍生分裂来最好地解释。在其他亚洲colobines中看到的人类1号和19号染色体的片段化和关联，但在非洲colobines中没有，最好解释为连接所有亚洲colobines的衍生相互易位。与人类1号和19号染色体同源的四个片段在N. laruatus的第6号染色体是相互易位的结果，随后是臂间倒位。N. laruatus与Tracypithecus共享这种臂间倒位，但不与Pygathrix共享。这种倒位显然是在尾须猿分化后将鼻猿和粗尾猿联系在一起的。核学资料支持了亚洲疣鼻猴包括N.幼虫是单系的。它们有许多共同的染色体特征，将它们与非洲的colobines分开。杂交模式也表明，Nasalis是嵌套在亚洲Colobines和股票的共同血统与其他亚洲colobines后的分歧Pygathrix的一段时间。(C)2003 Wiley-Liss，Inc.
The exceptional diploid number (2n = 48) of the proboscis monkey (Nasalis laruatus) has played a pivotal role in phylogenies that view the proboscis monkey as the most primitive colobine, and a long-isolated genus of the group. In this report we used molecular cytogenetic methods to map the chromosomal homology of the proboscis monkey in order to test these hypotheses. Our results reveal that the N. laruatus karyotype is derived and is not primitive in respect to other colobines (2n = 44) and most other Old World monkeys. The diploid number of 2n = 48 can be best explained by derived fissions of a segment of human chromosomes 14 and 6. The fragmentation and association of human chromosomes 1 and 19 as seen in other Asian colobines, but not in African colobines, is best explained as a derived reciprocal translocation linking all Asian colobines. The alternating hybridization pattern between four segments homologous to human chromosomes 1 and 19 on N. laruatus chromosome 6 is the result of the reciprocal translocation followed by a pericentric inversion. N. laruatus shares this pericentric inversion with Trachypithecus, but not with Pygathrix. This inversion apparently links Nasalis and Trachypithecus after the divergence of Pygathrix. The karyological data support the view that Asian colobines, including N. larvatus, are monophyletic. They share many linking karyological features separating them from the African colobines. The hybridization pattern also suggests that Nasalis is nested within Asian Colobines and shares a period of common descent with other Asian colobines after the divergence of Pygathrix. (C) 2003 Wiley-Liss, Inc.