Early constraint-induced movement therapy promotes functional recovery and neuronal plasticity in a subcortical hemorrhage model rat
Early constraint-induced movement therapy promotes functional recovery and neuronal plasticity in a subcortical hemorrhage model rat
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DOI：
10.1016/j.bbr.2015.02.022
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发表时间：
2015-05-01
影响因子：
2.7
通讯作者：
Hida, Hideki
中科院分区：
文献类型：
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作者：
Ishida, Akimasa;Misumi, Sachiyo;Hida, Hideki
强制性运动疗法（CIMT）可促进偏瘫中风（包括脑出血）后受损前肢的功能恢复。我们使用大鼠皮质下出血模型来比较ICH后早期或晚期给予CIMT的效果。通过将胶原酶注射到内囊附近的苍白球中，然后强迫大鼠在损伤后1天（早期CIMT）或17天（晚期CIMT）开始使用受影响的前肢7天来制作大鼠模型。早期CIMT后，前肢功能在熟练伸展试验和阶梯试验中有所恢复，而晚期CIMT或非CIMT对照组无明显恢复。早期CIMT与更多数量的AFosB阳性细胞在同侧感觉运动皮层层和V。此外，我们发现的生长相关基因的表达脑源性神经营养因子（BDNF）和生长相关蛋白43（GAP-43），和丰富的树突状树枝状锥体神经元的感觉运动区。在对侧皮质中未检测到类似结果。与早期CIMT相反，晚期CIMT未能诱导任何可塑性变化。我们的结论是，CIMT诱导分子和形态学可塑性在同病灶感觉运动皮层，并促进更好的功能恢复时，立即开始出血后。(C)2015爱思唯尔B.V.保留所有权利。
Constraint-induced movement therapy (CIMT) promotes functional recovery of impaired forelimbs after hemiplegic strokes, including intracerebral hemorrhage (ICH). We used a rat model of subcortical hemorrhage to compare the effects of delivering early or late CIMT after ICH. The rat model was made by injecting collagenase into the globus pallidus near the internal capsule, and then forcing rats to use the affected forelimb for 7 days starting either 1 day (early CIMT) or 17 days (late CIMT) after the lesion. Recovery of forelimb function in the skilled reaching test and the ladder stepping test was found after early-CIMT, while no significant recovery was shown after late CIMT or in the non-CIMT controls. Early CIMT was associated with greater numbers of AFosB-positive cells in the ipsi-lesional sensorimotor cortex layers and V. Additionally, we found expression of the growth-related genes brain-derived neurotrophic factor (BDNF) and growth-related protein 43 (GAP-43), and abundant dendritic arborization of pyramidal neurons in the sensorimotor area. Similar results were not detected in the contra-lesional cortex. In contrast to early CIMT, late CIMT failed to induce any changes in plasticity. We conclude that CIMT induces molecular and morphological plasticity in the ipsi-lesional sensorimotor cortex and facilitates better functional recovery when initiated immediately after hemorrhage. (C) 2015 Elsevier B.V. All rights reserved.