Production of lipids and formation and mobilization of lipid bodies in Chlorella vulgaris
Production of lipids and formation and mobilization of lipid bodies in Chlorella vulgaris
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DOI：
10.1007/s10811-012-9889-y
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发表时间：
2013-04-01
影响因子：
3.3
通讯作者：
Zachleder, Vilem
中科院分区：
文献类型：
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作者：
Pribyl, Pavel;Cepak, Vladislav;Zachleder, Vilem
我们研究了在自养生长的培养物中，在脂质诱导条件下，小球藻CCALA 256中脂质体的形成。我们发现，细胞分裂停止后耗尽硝酸盐从生长培养基中培养的第一天内。非分裂细胞的生长随后导致脂质的快速积累。我们详细描述了生物起源和动员的脂质体，使用荧光和透射电子显微镜。小脂质体在胞质溶胶中形成后很快融合，最终形成一个巨大的脂质体。在稳定生长期，脂质以占据大部分细胞体积的大脂质体的形式存在。补充氮后，脂质含量迅速下降，在24小时内，大的脂质体破碎成较小的。这种细胞脂质储存的动员不依赖于光而发生。
We investigated the formation of lipid bodies in the microalga Chlorella vulgaris CCALA 256 under lipid-induction conditions in autotrophically grown cultures. We found that cell division ceased after depletion of nitrates from the growth medium within the first days of cultivation. The growth of non-dividing cells subsequently led to the rapid accumulation of lipids. We describe in detail both biogenesis and mobilization of lipid bodies using fluorescence and transmission electron microscopy. Small lipid bodies fused very soon after their creation in the cytosol, forming, eventually, one huge lipid body. In stationary growth phase, lipids were present in the form of a large lipid body occupying most of the cell volume. After replenishment of nitrogen, lipid content decreased rapidly and, within 24 hours, the large lipid body was fragmented into smaller ones. This mobilization of the cellular lipid store occurred independently of light.