IL28B Gene Polymorphism SNP rs8099917 Genotype GG Is Associated with HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) in HTLV-1 Carriers
IL28B Gene Polymorphism SNP rs8099917 Genotype GG Is Associated with HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) in HTLV-1 Carriers
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DOI：
10.1371/journal.pntd.0003199
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发表时间：
2014-09-01
影响因子：
3.8
通讯作者：
Casseb, Jorge
中科院分区：
文献类型：
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作者：
Assone, Tatiane;de Souza, Fernando Vieira;Casseb, Jorge
背景：IL28B的多态性在某些病毒感染的发病机制中被认为是重要的。本研究的目的是评估IL28B基因多态性（SNP rs8099917和SNP rs12979860）是否与HAM/TSP相关。方法：229例受试者根据神经系统状况分为两组：I组（136例无症状HTLV-1携带者）和II组（93例HAM/TSP患者）。利用StepOnePlus实时荧光定量PCR系统对il28b基因区域rs8099917和rs12979860 snp进行分析。结果：包括性别、年龄和htlm -1 DNA前病毒载量在内的多变量模型分析显示，rs12979860基因型CT （OR = 2.03, IC95% = 0.96-4.27）和rs8099917基因型GG （OR = 7.61, IC95% = 1.82-31.72）中IL28B多态性与HAM/TSP预后独立相关。结论：rs8099917基因型GG和rs12979618基因型CT的受试者可能对HTLV-1感染表现出明显的免疫应答。因此，建议对所有htlv -1感染的受试者进行IL28B多态性的搜索，以监测其疾病发展的风险似乎是合理的；然而，由于这是文献中对这一发现的首次描述，我们应该首先在更多的htlv -1感染者中重复这项研究，以加强我们的结果已经提供的证据。
Background: The polymorphisms of IL28B have been described as important in the pathogenesis of infections caused by some viruses. The aim of this research was to evaluate whether IL28B gene polymorphisms (SNP rs8099917 and SNP rs12979860) are associated with HAM/TSP.Methods: The study included 229 subjects, classified according to their neurological status in two groups: Group I (136 asymptomatic HTLV-1 carriers) and Group II (93 HAM/TSP patients). The proviral loads were quantified, and the rs8099917 and rs12979860 SNPs in the region of IL28B-gene were analyzed by StepOnePlus Real-time PCR System.Results: A multivariate model analysis, including gender, age, and HTLV-1 DNA proviral load, showed that IL28B polymorphisms were independently associated with HAM/TSP outcome in rs12979860 genotype CT (OR = 2.03; IC95% = 0.96-4.27) and in rs8099917 genotype GG (OR = 7.61; IC95% = 1.82-31.72).Conclusion: Subjects with SNP rs8099917 genotype GG and rs12979618 genotype CT may present a distinct immune response against HTLV-1 infection. So, it seems reasonable to suggest that a search for IL28B polymorphisms should be performed for all HTLV-1-infected subjects in order to monitor their risk for disease development; however, since this is the first description of such finding in the literature, we should first replicate this study with more HTLV-1-infected persons to strengthen the evidence already provided by our results.