Experimental infection of cattle and goats with a foot-and-mouth disease virus isolate from the 2010 epidemic in Japan
Experimental infection of cattle and goats with a foot-and-mouth disease virus isolate from the 2010 epidemic in Japan
复制标题
DOI：
10.1007/s00705-014-2135-y
复制
发表时间：
2014-11-01
影响因子：
2.7
通讯作者：
Kanno, Toru
中科院分区：
文献类型：
--
作者：
Onozato, Hiroyuki;Fukai, Katsuhiko;Kanno, Toru
在这项研究中，我们使用2010年日本流行的口蹄疫病毒（FMDV）分离株对牛和山羊进行了实验性感染，以分析动物的临床表现，病毒脱落模式和抗体应答。我们发现FMDV O/JPN/2010分离株在牛和山羊中具有毒性，产生临床症状，通过直接接触在同一物种内有效传播，并且在牛中具有持续感染性。对感染该分离株的牛和山羊组织中病毒RNA水平的定量分析表明，咽部区域是FMDV O/JPN/2010的重要主要靶标。本研究中FMDV O/JPN/2010的病毒载量、排泄和传播的时间过程数据是提供流行病学调查和疾病控制相关风险分析所必需的定量数据的关键。
In this study, we carried out experimental infections in cattle and goats using a foot-and-mouth disease virus (FMDV) isolate from the 2010 epidemic in Japan to analyze clinical manifestations, virus-shedding patterns and antibody responses in the animals. We found that the FMDV O/JPN/2010 isolate is virulent in cattle and goats, produces clinical signs, is spread efficiently by direct contact within the same species, and is persistently infectious in cattle. Quantitative analysis of levels of viral RNA in the tissues of cattle and goats infected with the isolate showed that the pharyngeal region is an important major target of the FMDV O/JPN/2010. Time course data of viral loads, excretion and transmission of the FMDV O/JPN/2010 in this study are key in providing quantitative data essential for epidemiological investigation and risk analysis in relation to disease controls.