Transition from non-motile behaviour to directed migration during early PGC development in zebrafish
Transition from non-motile behaviour to directed migration during early PGC development in zebrafish
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DOI：
10.1242/jcs.02522
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发表时间：
2005-09-01
影响因子：
4
通讯作者：
Raz, E
中科院分区：
文献类型：
--
作者：
Blaser, H;Eisenbeiss, S;Raz, E
斑马鱼原始生殖细胞(PGCs)的迁移是由SDF-1a引导的，是趋化因子引导的细胞迁移的模型。虽然斑马鱼PGC的发育和迁移已经从原肠中期开始进行了非常详细的研究，当时细胞表现出引导的主动迁移[受精后7-8小时(HPF)]，但还没有对早期阶段进行研究。在这里，我们展示了PGC在离散的成熟步骤后获得了对趋化因子的应答能力。使用新基因askopos的启动子和Nanos1的RNA元件来驱动GFP在PGCs中的表达，我们发现在它们指定(约3HPF)后，PGCs立即呈现出简单的细胞形态。这一阶段之后是一个阶段，在这个阶段，细胞具有复杂的形态，但它们既不改变位置，也不对SDF-1a做出反应。在第三阶段，随着PGCs对分泌趋化因子SDF-1a的体细胞提供的定向信号做出反应，发生了向“迁移阶段”的转变。这种转变依赖于合子转录和RNA结合蛋白Dead end的功能，并与细胞黏附分子E-cadherin的下调有关。这些独特的形态和分子变化可能代表着对发育和疾病至关重要的类似过程中的普遍发生。
The migration of zebrafish primordial germ cells (PGCs) is directed by SDF-1a and serves as a model for long-range chemokine-guided cell migration. Whereas the development and migration of zebrafish PGCs have been studied in great detail starting at mid-gastrulation stages when the cells exhibit guided active migration [7-8 hours post fertilization (hpf)], earlier stages have not yet been examined. Here we show that the PGCs acquire competence to respond to the chemokine following discrete maturation steps. Using the promoter of the novel gene askopos and RNA elements of nanos1 to drive GFP expression in PGCs, we found that immediately after their specification (about 3 hpf) PGCs exhibit simple cell shape. This stage is followed by a phase at which the cells assume complex morphology yet they neither change their position nor do they respond to SDF-1a. During the third phase, a transition into a 'migratory stage' occurs as PGCs become responsive to directional cues provided by somatic cells secreting the chemokine SDF-1a. This transition depends on zygotic transcription and on the function of the RNA-binding protein Dead end and is correlated with down regulation of the cell adhesion molecule E-cadherin. These distinctive morphological and molecular alterations could represent a general occurrence in similar processes critical for development and disease.