SELECTION AND CHARACTERIZATION OF BOVINE AORTIC ENDOTHELIAL CELLS
SELECTION AND CHARACTERIZATION OF BOVINE AORTIC ENDOTHELIAL CELLS
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DOI：
10.1007/bf02616210
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发表时间：
1978-01-01
期刊：
影响因子：
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通讯作者：
SCHWARTZ, SM
中科院分区：
文献类型：
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作者：
SCHWARTZ, SM
本文报道了牛主动脉内皮（BAE）的分离、筛选和长期传代技术。一种[3H]胸腺嘧啶选择技术通过污染平滑肌细胞来限制培养物的过度生长。所得到的培养物可以传代35-40倍的繁殖寿命，并在此期间保持稳定，正常的核型。尽管这些培养物在密度抑制的生长状态下达到了稳定的单层，但融合后的细胞显示出第二种生长模式的焦点区域，称为发芽。只有在高密度培养1-2周时才会出现这种情况。超微结构分析以及内皮细胞（因子VIII）和平滑肌细胞（肌动蛋白）标记物的免疫荧光研究表明，这种现象不是由于剩余平滑肌细胞群过度生长，而可能代表内皮细胞本身的第二种生长模式。
Techniques for isolation, selection and long-term passage of bovine aortic endothelium (BAE) are reported. A [3H]thymidine-selection technique was developed to limit overgrowth of cultures by contaminating smooth-muscle cells. The resulting cultures could be passaged for a replicative life span of 35-40 doublings and maintained a stable, normal karyotype throughout this period. Despite the fact that these cultures reached a stable monolayer with density-inhibited growth state, postconfluent cells showed focal areas of a 2nd growth pattern called sprouting. This was seen only when cultures were maintained at high densities for periods of 1-2 wk. Ultrastructural analysis, and immunofluorescence studies with markers for endothelial cells (factor VIII) and smoothmuscle cells (actin) indicate that the phenomenon is not due to overgrowth of a residual population of smooth-muscle cells but may represent a 2nd growth pattern of the endothelial cells themselves.