Sterilization, storage stability and in vivo biocompatibility of poly(trimethylene carbonate)/poly(adipic anhydride) blends
Sterilization, storage stability and in vivo biocompatibility of poly(trimethylene carbonate)/poly(adipic anhydride) blends
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DOI：
10.1016/s0142-9612(99)00268-9
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发表时间：
2000-05-01
期刊：
影响因子：
14
通讯作者：
Lundgren, BO
中科院分区：
文献类型：
--
作者：
Edlund, U;Albertsson, AC;Lundgren, BO
聚碳酸三亚甲酯(PTMC)和聚己二酸酐(PAA)的生物可降解共混物已被证明是体外可控释药聚合物的有力候选者。我们现在报告这些基质的稳定性、灭菌性和体内局部组织反应。通过β辐射或环氧乙烷气体处理对共混基质进行灭菌，在不同的时间和温度下储存，并分析物理化学性质的变化。测定了不同相对湿度和不同贮藏时间下的吸湿率。灭菌程序导致基质的水解。环氧乙烷气体灭菌对基质性能的影响明显大于辐射处理。随着共混物组成的变化，共混物的结晶度和相对分子质量都有所下降，这反映了共混物的降解开始。在空气中储存时，观察到类似的基质降解开始。共混物在Ar气氛下储存后，其物化性能得到了很好的保存。术后1个月评价PTMC/PAA植入物在兔眼前房内的生物相容性。在选定的术后时间点，分析房水中的白细胞，并测量角膜厚度。结果表明，富含PTMC的基质具有良好的生物相容性，而快速侵蚀的富含PAA的基质由于降解产物的突然释放而引起炎症反应。(C)2000爱思唯尔科学有限公司。保留所有权利。
Biodegradable blends of poly(trimethylene carbonate) (PTMC) and poly(adipic anhydride) (PAA) have been proven to be strong candidates for controlled drug delivery polymers in vitro. We now report on the stability, sterilizability and in vivo local tissue response of these matrices. Blend matrices were sterilized by beta-radiation or ethylene oxide gas treatment, stored at different times and temperatures, and analyzed for changes in physicochemical properties. Moisture uptake at different relative humidities and storage times was determined. Sterilization procedures induced hydrolysis of the matrices. Ethylene oxide gas sterilization had a significantly more marked effect upon the matrix properties than radiation treatment. The onset of degradation was reflected in a decrease of crystallinity and molecular weight along with a change of blend composition. A similar onset of matrix degradation was observed upon storage in air. The physicochemical properties of the blends were well preserved upon storage under argon atmosphere. Biocompatibility of PTMC/PAA implants was assessed in the anterior chamber of rabbits eyes for 1 month. At selected post-operative time points, aqueous humor was analyzed for white blood cells and the corneal thickness was measured. The results suggest good biocompatability of PTMC-rich matrices, whereas fast eroding PAA-rich matrices caused inflammatory responses, due to a burst release of degradation products. (C) 2000 Elsevier Science Ltd. All rights reserved.