Infection of immunocompetent mice with acid-water-pretreated Cryptosporidium parvum results in weight loss, and intestinal (structural and physiological) alterations.
Infection of immunocompetent mice with acid-water-pretreated Cryptosporidium parvum results in weight loss, and intestinal (structural and physiological) alterations.
复制标题
用酸水预处理的小隐孢子虫感染免疫活性小鼠会导致体重减轻和肠道（结构和生理）改变。
DOI：
10.1007/s00436-007-0785-3
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发表时间：
2008
影响因子：
2
通讯作者：
Robinson,Prema
中科院分区：
文献类型：
--
作者：
Garza,Armandina;Castellanos-Gonzalez,Alejandro;Castenallos-Gonzalez,Alejandro;Griffiths,Jeffrey;Robinson,Prema
Cryptosporidiosis, caused byCryptosporidium, causes self-limited diarrhea in normal hosts but may cause life-threatening diarrhea in immunocompromised persons.Cryptosporidium-induced manifestations, including weight-loss and intestinal physiological alterations are not noted in adult immunocompetent mice. So far, studies that have been used to test the therapeutic efficacy of drugs have been performed using various immunocompromised animal models. There is an urgent need of an immunocompetent small animal model that portraysCryptosporidium-induced manifestations. In the current studies, we have compared twoCryptosporidium parvumpretreatment methods, we have hence used sodium hypochlorite or acidic water to treatCryptosporidium parvum, followed by infection by oral gavage in adult immunocompetent C57BL6 mice. We demonstrated manifestations such as weight loss, intestinal structural and physiological alterations such as intestinal, villi blunting, and glucose malabsorption (as studied by the Ussing chamber technique) only in response to infection withC. parvumthat has been treated with acidic water and not with sodium hypochlorite. These novel studies reveal that acidic water treatment ofC. parvumresults in manifestations of cryptosporidiosis in otherwise resistant immunocompetent mice. The current studies open up possibilities of using the normal immunocompetent mice model to test therapeutic drugs against cryptosporidiosis.
