Structure and Biosynthesis Mechanism of Rubber from Fungi
Structure and Biosynthesis Mechanism of Rubber from Fungi
复制标题
真菌橡胶的结构与生物合成机制
DOI：
10.5254/1.3538238
复制
发表时间：
1990
影响因子：
1.5
通讯作者：
K. Hatada
中科院分区：
文献类型：
--
作者：
Yasuyuki Tanaka;Makio Mori;K. Ute;K. Hatada
摘要末端单元的结构和异戊二烯单元的排列为真菌橡胶形成的起始和终止机制提供了确凿的证据。如图4所示，聚合估计从反式，反式-法呢基焦磷酸开始，并通过IPP的连续缩合以形成顺式构型的异戊二烯单元而进行。推测终止是通过聚异戊二烯焦磷酸酯（PIP-PP）的直接酯化或PIP-PP的脱磷酸化随后与脂肪酸酯化而发生。值得注意的是，通过从L.在冰箱中储存2-3天后，不加盐。在这些样品中，还检测到未鉴定的13 C-NMR信号。这些结果表明，PIP-PP的直接酯化更可能作为终止反应。它...
Abstract The structure of both terminal units and alignment of the isoprene units give conclusive evidence for the initiation and termination mechanisms of rubber formation in fungi. As shown in Figure 4, the polymerization is estimated to start from trans, trans-farnesyl pyrophosphate and proceeds by successive condensation of IPP to form isoprene units in the cis configuration. The termination is presumed to occur by direct esterification of polyisoprenyl pyrophosphate (PIP-PP) or dephosphorylation of PIP-PP followed by esterification with fatty acids. It is remarkable that the hydroxyl terminal group corresponding to the saponification product from the esterified terminal group was observed in the rubber samples by extraction from sporophores of L. volemus after storage for 2–3 days in a refrigerator without the addition of salt. In these samples, unidentified 13C-NMR signals were also detected. These findings suggest that the direct esterification of PIP-PP is more likely as a termination reaction. It...